声门上型喉鳞状细胞癌诱导化疗潜在靶向基因的初步分析

A preliminary analysis on potentially targeted genes of induced chemotherapy in supraglottic laryngeal squamous cell carcinoma

目的 利用基因表达谱芯片,寻找声门上型喉鳞状细胞癌（简称声门上型喉癌）紫杉醇＋顺铂＋5氟尿嘧啶（TPF）诱导化疗敏感性的差异表达基因,探讨声门上型喉癌诱导化疗潜在的功能性靶向基因.方法 选取11例声门上型喉癌患者治疗前的新鲜肿瘤标本,分为化疗敏感组（7例）和不敏感组（4例）.采用Illumina人类全基因组表达谱芯片检测两组的表达差异,并对差异基因进行生物信息学在线分析.结果 共筛选出1 554个差异基因.经GO数据库分析,功能上调的集合主要包括：生物黏附、免疫系统发育及干细胞增殖等;下调的集合包括：细胞连接结构、磷代谢过程及细胞形态发生相关分化等集合.经KEGG数据库分析,上调通路主要有：P53通路、细胞黏附分子、Ras信号通路等.下调通路主要有：黏着斑、胞吞作用、ErbB信号通路等.其中MAPK10、PIK3R5、JUN等基因表达有显著差异且有生物学意义.结论 通过对化疗敏感与不敏感组的差异表达研究,功能核心基因MAPK10、PIK3R5、JUN等可能是声门上型喉癌诱导化疗敏感性相关的潜在功能性基因.

Objective To analyze the differentially expressed genes produced by paclitaxel,cisplatin and 5-fluorouracil（TPF） regimen induction chemotherapy and the potentially functionally targeted genes of the induc ed chemotherapy in supraglottic laryngeal squamous cell carcinoma.Methods A total of 11 tissue samples from patients diagnosed as supraglottic carcinoma who didnt receive any treatment before were analyzed with microarray.The patients were divided into two groups based on their responses to the induction chemotherapy：7 were sensitive to chemotherapy and 4 were non-sensitive.Gene expressions were detected by Illumina Human HT-12 BeadChip.The bioinformatics analysis online was used to analyze the differentially expressed genes.Results A total of 1 554 differentially expressed genes related to chemosensitivity were found.Analyzed with GO database,the up-regulated genes included the functional sets of biological adhesion,immune system development and stem cell proliferation,and the down-regulated genes included the functional sets of cell junction organization,phosphorus metabolic process and cell morphogenesis involved in differentiation.Analyzed with KEGG database,the up-regulated pathways included p53,cell adhesion and Ras signaling pathways,and the down-regulated pathways included focal adhesion,endocytosis and ErbB signaling pathways.There were statistically significant differences in the expressions of MAPK10,PIK3R5 and JUN genes,which had biological significance,between sensitive patients and non-sensitive patients.Conclusion MAPK10,PIK3R5 and JUN may be considered as potentially functional genes of the induced chemotherapy in supraglottic carcinoma.