摘要
目的:研究北豆根根茎中双苄基异喹啉类生物碱成分。方法:利用硅胶柱色谱、高效液相色谱等色谱技术分离得到单体化合物,利用NMR鉴定化合物结构。结果:从北豆根根茎中分离得到7个双苄基异喹啉类生物碱,经波谱分析分别鉴定为新四川轮环藤因(1),华东唐松草碱(2),盾叶轮环藤碱(3),高芳香花桂林碱(4),四川轮环藤宁碱(5),四川轮环藤因(6)和裂环异粉防己碱(7)。化合物1~3和5具有较好的抑制人肝癌细胞(Hep G-2)增殖的活性,IC50值分别为0.58、0.73、0.90、1.66μmol·L^(-1)。结论:化合物1~7为从该植物中首次分离得到。
Objective: To study the chemical constituents of the rhizome of Menispermum dauricum. Methods: The compounds were separated and purified by column chromatographic and HPLC chromatographic methods. Structure analyses were carried out by nuclear magnetic resonance (NMR) technology. Results: Seven bisbenzylisoquinoline alkaloids named neosutchuenenine ( 1), thalifortine ( 2 ), cycleapeltine (3), homoaromoline (4), sutchueneneonine ( 5 ), sutchuenenine (6), and secoisotetrandrine (7) were isolated from the rhizome of M. dauricum. Compounds 1-3 and 5 exhibited cytotoxic activity against HepG-2 with IC50 values of 0. 58, 0. 73, 0. 90, and 1.66 μmol·L^-1, respectively. Conclusion: Compounds 1-7 were obtained from the plant for the first time.
出处
《中国现代中药》
CAS
2016年第8期951-955,共5页
Modern Chinese Medicine
基金
国家自然科学基金(81274035)
黑龙江中医药大学创新人才项目(2012)
黑龙江中医药大学佳木斯学院自主课题
关键词
北豆根
双苄基异喹啉类生物碱
细胞毒活性
Menispermum dauricum
bisbenzylisoquinoline alkaloids
cytotoxic activity