北豆根根茎中双苄基异喹啉类生物碱成分研究

Bisbenzylisoquinoline Alkaloids from the Rhizome of Menispermum dauricum

目的:研究北豆根根茎中双苄基异喹啉类生物碱成分。方法:利用硅胶柱色谱、高效液相色谱等色谱技术分离得到单体化合物,利用NMR鉴定化合物结构。结果:从北豆根根茎中分离得到7个双苄基异喹啉类生物碱,经波谱分析分别鉴定为新四川轮环藤因(1),华东唐松草碱(2),盾叶轮环藤碱(3),高芳香花桂林碱(4),四川轮环藤宁碱(5),四川轮环藤因(6)和裂环异粉防己碱(7)。化合物1~3和5具有较好的抑制人肝癌细胞(Hep G-2)增殖的活性,IC50值分别为0.58、0.73、0.90、1.66μmol·L^(-1)。结论:化合物1~7为从该植物中首次分离得到。

Objective： To study the chemical constituents of the rhizome of Menispermum dauricum. Methods： The compounds were separated and purified by column chromatographic and HPLC chromatographic methods. Structure analyses were carried out by nuclear magnetic resonance （NMR） technology. Results： Seven bisbenzylisoquinoline alkaloids named neosutchuenenine （ 1）, thalifortine （ 2 ）, cycleapeltine （3）, homoaromoline （4）, sutchueneneonine （ 5 ）, sutchuenenine （6）, and secoisotetrandrine （7） were isolated from the rhizome of M. dauricum. Compounds 1-3 and 5 exhibited cytotoxic activity against HepG-2 with IC50 values of 0. 58, 0. 73, 0. 90, and 1.66 μmol·L^-1, respectively. Conclusion： Compounds 1-7 were obtained from the plant for the first time.