交联酶聚集体转化葡萄糖制备葡萄糖酸的研究

Bioconversion of glucose to glucose acid by cross-linked enzyme aggregates

共交联葡萄糖氧化酶-过氧化氢酶具有较好的耐酸性,可以直接转化葡萄糖制备葡萄糖酸。利用正交实验优化其转化条件:反应温度为31℃,转速为150 r/min,加酶质量分数为2.0%,底物质量浓度为150 g/L。发酵罐放大实验显示其转化率可以达到97.2%,并且交联酶的稳定性较好,可以有效的连续使用3个批次,平均转化率为93.8%。利用葡萄糖氧化酶-过氧化氢酶共交联聚集体制备葡萄糖酸是一种简便、高效的方法。

The combination of crosslinked glucose oxidase and catalase aggregates has characteristic of good acid resistance,which can directly convert glucose to glucose acid. The optimal conversion conditions are obtained as follows by using orthogonal experiment：31℃ of the reaction temperature,2% of the amount of enzyme,150 r / min of the speed and 150 g / L of the concentration of the substrate. Under the optimal conditions,the conversion of amplification experiment in fermentor is 97. 2%. The cross-linked enzyme is stable,which could be used for three consecutive cycles with the average conversion of 93. 8%. It is a simple and effective method to prepare glucose acid by combining crossinked glucose oxidase with catalase aggregates.