汉防己碱对牛血清白蛋白致大鼠肝纤维化的治疗作用

Tetrandrine in treatment of liver fibrosis induced by bovine serum albumin in mice

目的研究汉防己碱对牛血清白蛋白(bovine serum albumin,BSA)致大鼠肝纤维化的治疗作用。方法 90只SD大鼠随机分为6组,即正常组、模型组、秋水仙碱阳性对照组(0.1 mg/kg)、汉防己碱高剂量组(8 mg/kg)、汉防己碱中剂量组(4 mg/kg)和汉防己碱低剂量组(2 mg/kg),每组15只。造模成功后连续80天灌胃给予大鼠汉防己碱。末次给药后腹主动脉采血并取肝脏活体,检测大鼠血清学指标(ALT、AST、TP、ALB、LN、HA、PC-Ⅲ及Ⅳ-C胶原)并观察其肝脏病理组织学变化情况。结果与模型组相比,汉防己碱高剂量组、中剂量组及秋水仙碱组大鼠相关血清学水平得到显著改善,其中治疗后汉防己碱高剂量组LN、HA、PC-Ⅲ及Ⅳ-C胶原水平分别为(45.36±1.92)ng/ml、(232.54±23.71)ng/ml、(30.69±2.93)ng/ml、(29.20±2.82)ng/ml;汉防己碱中剂量组LN、HA、PC-Ⅲ及Ⅳ-C胶原水平分别为(52.17±2.99)ng/ml、(283.12±23.19)ng/ml、(34.92±2.72)ng/ml及(34.81±2.92)ng/ml,均显著优于模型组。病理学结果显示,与模型组比较,汉防己碱高、中剂量组及秋水仙碱组大鼠的肝细胞水肿、变性和坏死情况均显著减轻。结论汉防己碱具有较好的保护肝细胞和治疗肝纤维化的作用。

Objective To observe the therapeutical effect of tetrandrine on liver fibrosis induced by bovine serum albumin（BSA） in mice. Methods Total of 90 SD mice were divided into 6 groups： healthy control group, model group, high dose of tetrandrine group（8 mg/kg）, middle dose of tetrandrine group（4 mg/kg）, low dose of tetrandrine group（2 mg/kg）, model group and colchicine group（0.1 mg/kg）, 15 mice in each group. After the mice models were made, the high, middle and low dose of tetrandrine group were given by gavage for 80 days, abdominal aortic blood and the livers were taken after the last administration. The serological indicators（ALT, AST, TP, ALB, LN, HA, PC-Ⅲ and Ⅳ-C） were detected and liver pathological histology changes were observed. Results Compared with the model group, the serological indicators in colchicine group, high and middle dose of tetrandrine group decreased significantly. After treatment, the levels of LN, HA, PC-Ⅲ and Ⅳ-C were（45.36 ± 1.92） ng/ml,（232.54 ± 23.71） ng/ml,（30.69 ± 2.93） ng/ml,（29.20 ± 2.82） ng/ml in the high dose of tetrandrine group and（52.17 ± 2.99） ng/ml,（283.12 ± 23.19） ng/ml,（34.92 ± 2.72） ng/ml,（34.81 ± 2.92） ng/ml in the middle dose of tetrandrine group, respectively, which were obviously superior to the model group. Compared with the model group, the degrees of cellular swelling, degeneration and necrosis of the liver in high and middle dose of tetrandrine group and colchicine group relieved obviously. Conclusion Tetrandrine can protect liver cells and can also prevent and treat liver fibrosis of rats.