Glypican-3对HepG2肝癌细胞增殖及侵袭转移的影响

Effects of Glypican-3 on proliferation, invasiveness and migratory metastasis of HepG2 cells

目的探讨磷脂酰肌醇蛋白多糖-3(Glypican-3,GPC-3)在Hep G2肝癌细胞增殖及侵袭转移中的作用。方法构建并筛选沉默GPC-3最有效的shRNA,转染HepG2细胞,Western blot分析GPC-3蛋白表达水平;MTT、划痕实验和Transwell小室实验分别检测GPC-3基因沉默对HepG2细胞增殖、转移和侵袭能力的影响。结果 GPC-3-shRNA-1干扰效果最好,对GPC-3表达抑制率为77.7%;GPC-3沉默后HepG2细胞增殖显著受到抑制(P=0.014),细胞转移和侵袭能力显著减弱,侵袭抑制率为52.4%。结论 GPC-3基因沉默后Hep G2细胞增殖、转移和侵袭能力显著受抑制,提示GPC-3在肝癌的发生发展中起关键作用,可能成为肝癌靶向治疗的目标。

Objective To investigate the effects of Glypican-3 on proliferation, invasiveness and migratory metastasis of HepG2 cells. Methods High effective GPC-3-shRNA was obtained by construction and screening. The expression of GPC-3 was analyzed by western blot after transfected into HepG2 cells. The effects of gene silence of GPC-3 on proliferation, invasiveness and migratory metastasis of HepG2 cells were analyzed by MTT, cell scratch test and transwell chamber system, respectively. Results The most effective sh RNA inhibited 77.7% expression of GPC-3. The proliferation of HepG2 cells were significantly inhibited after GPC-3 silence（P = 0.014）. The migratory metastasis and invasiveness with reduced significantly, and the invasion inhibition rate was 52.4%. Conclusion The proliferation, invasiveness and migratory metastasis of HepG2 cells were significantly inhibited after GPC-3 silence, which suggests that GPC-3 may play an important role in the development of hepatic carcinoma and contribute to targeted therapy for hepatic carcinoma.