GPC3和AFP联合检测在原发性肝癌诊断中的价值

Values of combined detection of GPC3 and AFP in diagnosis of primary liver cancer

目的探讨磷脂酰肌醇蛋白聚糖3(glypican-3,GPC3)和甲胎蛋白(alpha fetoprotein,AFP)联合检测在原发性肝癌(primary liver cancer,PLC)诊断中的价值。方法本研究共纳入PLC患者107例,普通肝病患者49例,其他肿瘤患者45例,健康对照60例,AFP采用微粒子化学发光法,用美国雅培ARCHITECT i2000SR全自动化学发光仪进行定量检测;GPC3采用酶联免疫吸附试验(ELISA法)进行定量检测。结果 PLC组血清GPC3水平均显著高于普通肝病组、其他肿瘤组和健康对照组,差异有统计学意义(P=0.00);GPC3、AFP及二者联合检测对诊断PLC的灵敏度分别为58.88%、62.62%、91.59%,其受试者工作特征曲线(ROC曲线)下面积(AUC)分别为0.71、0.73、0.92。结论GPC3和AFP联合检测能提高PLC诊断的灵敏度和诊断价值。

Objective To investigate the values of combined detection of GPC3 and AFP in diagnosis of primary liver cancer（PLC）. Methods Total of 107 patients with PLC, 49 patients with common liver disease, 45 patients with other cancer and 45 cases of healthy controls were selected. AFP was detected by Abbott ARCHITECT i2000 SR and GPC3 was detected by ELISA methods. Results GPC3 levels in PLC group was significantly higher than that of normal liver group, the other tumor group and healthy group, the differences were statistically significant（P = 0.00）. The sensitivity of GPC3, AFP and combined detection in diagnosis of PLC were 58.88%, 62.62%, 91.59% and the AUC were 0.71, 0.73, 0.92, respectively. Conclusion Combined detection of GPC3 and AFP can improve the sensitivity and diagnostic value of PLC diagnosis.