能谱CT评价兔VX2肝癌抗血管生成治疗的实验研究

Experimental Study on the Evaluation of Anti-Angiogenesis Therapy in Rabbit VX2 Liver Cancers by Spectrum CT

目的分析能谱CT对兔VX2肝癌模型抗血管生成疗效评估的价值。方法将开腹种植成功的68只肝癌模型,平均分为2组,对照组于移植瘤术后14 d开始,每天静脉注射和治疗组相同剂量安慰剂,生理盐水,连续注射7 d。治疗组每天静脉注射重组入血管内皮抑制素连续注射7 d。对建模成功的两组兔VX2肝移植瘤在种植后14 d、21 d均行能谱扫描。将重建图像数据传输到影像后处理工作站进行分析。在碘基图上定量测量肿瘤区域、邻近无瘤肝组织、腹主动脉的碘含量,分别计算标准化碘含量(NIC),比较治疗前后,肿瘤区域之间标准化碘含量的差异。结果治疗前,实验组、对照组肿瘤组织NIC均大于邻近无瘤肝组织,之间的差异具有统计学意义(P<0.05),而实验组和对照组之间无统计学差异。重组人血管内皮抑制素(Endostar,恩度)治疗后,治疗组肿瘤区域NIC小于对照组,并且小于治疗前肿瘤区域NIC,差异有统计学意义(P<0.05)。结论重组人血管内皮抑制素(恩度)表现出明显的抑制肿瘤血管生成作用,在化疗的过程可通过能谱扫描评价抗血管治疗的效果。

Objective To explore the effectiveness of application of the spectral CT for the treatment of endostatin of the rabbit VX2 liver cancer model. Methods The 68 rabbits with VX2 liver tumors were randomly and evenly divided into Control Group and Trial Group with 34 rabbits in either group. From the 14 d after tumor transplantation, Group A was injected with endostain at the dose of 0.3 mg/kg continuously for 7 d; while, Group B was injected with saline and endostain at the same dose of 0.3 mg/kg with Group A continuously for 7 d, The rabbits were scanned to obtain gemstone spectral images. The reconstructed image data were transmitted to the image processing workstation for analysis. The iodine content of the tumor, area without tumor and the abdominal aorta were measured in the iodine map to calculate NIC（Normalized Iodine Concentrations）. And the pre- and post-treatment NIC was compared. Results The NIC of the tumor tissue in both Group A and Group B were higher than the liver tissue without tumor, with statistically significant difference（P〈0.05）. After the endostatin Endostar treatment, the NIC of tumor of Trail Group was less than that of Control Group, and less than pre-trestment NIC, with statistically significant（P〈0.05）. Conclusion The endostain Endosar showed obvious antiangiogenic effects in spectrum CT scan to evaluate the tumor angiogenesis and the effect of the anti-angiogenesis therapy.