ISSR分子标记鉴定巴马香猪

Species Authentication of Bama Miniature Pig through Inter-simple Sequence Repeat

结合线粒体基因和ISSR两种DNA分子标记技术对巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪进行种类鉴定。研究从55条ISSR引物中筛选出1条带型清晰、重复性好的引物53可以鉴别巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪。巴马香猪与藏猪线粒体基因分析结果表明,藏猪具有特异的短串联重复片段"TGTGTACGCA",可用于进一步鉴别巴马香猪与藏猪。ISSR分子标记与聚类分析结果显示,引物53可以鉴别5种猪并成功鉴定了一份未知种类的巴马产猪肉样品。结果表明,ISSR分子标记与线粒体基因联合分析可以快速准确鉴别巴马香猪、环江香猪、藏猪、巴马本地土猪和成华猪。

In this study,species identification of Bama miniature pig was carried out by DNA fingerprinting of meats from Tibetan pig,Huanjiang miniature pig,Bama native pig,and Chenghua pig through inter-simple sequence repeat（ ISSR）and mitochondrial DNA（ mt DNA） analysis. One clear and repeatable primer pair named No. 53 was selected from 55 primers,which successfully separated Bama miniature pig from the other four pigs. To further differentiate Bama miniature pig and Tibetan pig,specific tandem repeat sequence of Tibetan pig‘TGTGTACGCA＇was found out in the mt DNA. According to the results of ISSR and UPGMA cluster analysis,primer No. 53 was able to attribute to the identification of the five different pigs and one unknown pig. Therefore,ISSR coupled with UPGMA and mt DNA analysis represented an effective tool in species identification.