含锌磷酸三钙陶瓷促进骨质疏松大鼠下颌骨极限缺损修复实验研究

Reconstruction of mandibular critical defects in ovariectomized rats with zinc-releasing calcium phosphate ceramics

目的评价含锌生物材料对于骨质疏松骨修复的作用。方法通过摘除成年雌性大鼠双侧卵巢(去势)的方法建立下颌骨骨质疏松动物模型,12周后检测模型成功后,建立下颌骨极限缺损模型,随后将动物随机分为3组。实验组植入含锌磷酸三钙陶瓷(Zn-TCP/HA),对照组植入双相磷酸钙陶瓷(β-TCP),空白对照组不植入材料。在植入材料12周后处死动物,进行组织学观察和生物力学检测。结果通过骨密度仪测试证实去势大鼠的下颌骨有骨质疏松发生;材料植入骨缺损部位12周后,通过影像学检查、生物力学检测以及组织学观察发现,实验组骨修复效果明显优于对照组和空白对照组。结论锌离子能够加速和改善骨质疏松骨缺损的修复。

Objective To evaluate the advancement of zinc for bone repair with osteoporosis. Methods The osteoporosis model have been established by ovariectomy （OVX）in female rats for 12 weeks. Then, critical defects （5 ram）were prepared in bilateral mandible with a trephine and the rats were divided into three groups as the repair methods, in which experimental group were restored with zinc-releasing calcium phosphate ceramic/hydroxyapatite （Zn-TCP/HA）, control group with 13-TCP and blank control group were sutured directly. After 12 weeks, improved bone repair and shorten healing time in the OVX rats would be tested through biomechanical tests, radiographic and histological examination. Resuits Osteoporosis in mandibular of female rats had been confirmed by bone density testified. Then, 12 weeks after im- plantation, experimental group showed better bone repair than control group and blank control group through biomeehani- cal tests, radiographic and histological examination. Conlusion Zn-releassing scaffolds would obviously improved bone regeneration of the critical-sized defect in OVX rats model and provides valuable information for the therapy of osteoporosis related diseases by tissue engineering approach.