槲皮素对去卵巢大鼠血清骨钙素和股骨Ⅰ型胶原蛋白水平的影响

Effect of quercetin on serum osteocalcin levels and femur typeⅠcollagen protein levels in the ovariectomized rats

目的:观察槲皮素对去卵巢大鼠血清骨钙素和股骨Ⅰ型胶原蛋白水平的影响。方法:将50只6月龄清洁级健康雌性SD大鼠随机分为假手术组、模型组、尼尔雌醇组、槲皮素高剂量组和槲皮素低剂量组,每组10只。假手术组大鼠开腹找到卵巢后即关闭腹腔,其余4组切除双侧卵巢。造模手术1周后,假手术组和模型组以生理盐水灌胃,每天1次,每次4 m L;尼尔雌醇组按1.5 mg·kg-1以尼尔雌醇灌胃,每周1次;槲皮素高剂量组和槲皮素低剂量组分别按200 mg·kg-1和100 mg·kg-1以槲皮素灌胃,每天1次。3个月后以酶联免疫吸附测定法测定各组大鼠血清骨钙素水平,以Western Blot法测定股骨干骺端Ⅰ型胶原蛋白水平。结果:至药物干预结束时,模型组、尼尔雌醇组及槲皮素高剂量组各有1只大鼠死亡,槲皮素低剂量组2只大鼠死亡,死亡原因均为肺部感染。5组大鼠血清骨钙素水平比较,差异有统计学意义[(2.562±0.671)ng·m L-1,(5.763±0.612)ng·m L-1,(2.674±1.170)ng·m L-1,(2.722±1.063)ng·m L-1,(3.574±1.914)ng·m L-1,F=98.527,P=0.000]。假手术组、尼尔雌醇组、槲皮素高剂量组、槲皮素低剂量组血清骨钙素水平均低于模型组(P=0.000,P=0.000,P=0.000,P=0.005);槲皮素高剂量组与尼尔雌醇组比较,差异无统计学意义(P=0.361);槲皮素低剂量组高于尼尔雌醇组(P=0.047);槲皮素低剂量组与槲皮素高剂组比较,差异无统计学意义(P=0.106)。5组大鼠股骨干骺端Ⅰ型胶原蛋白表达水平比较,差异有统计学意义(2.718±0.362,1.492±0.228,2.624±0.296,2.497±0.274,1.713±0.327,F=10.726,P=0.000)。假手术组、尼尔雌醇组、槲皮素高剂量组股骨Ⅰ型胶原蛋白表达水平均高于模型组(P=0.000,P=0.000,P=0.000);槲皮素低剂量组与模型组比较,差异无统计学意义(P=0.056);槲皮素高剂量组与尼尔雌醇组比较,差异无统计学意义(P=0.217);槲皮素低剂量组低于槲皮素高剂量组和尼尔雌醇组(P=0.000,P=0.000)。结论:槲皮素具有类雌激素样作用,可有效降低去卵巢大鼠的血清骨钙素水平、提高股骨Ⅰ型胶原蛋白水平,其作用效果与剂量有关。

Objective： To observe the effect of quercetin on serum osteocalcin levels and femur typeⅠ collagen protein levels in the ovariectomized rats. Methods： Fifty healthy 6-month-old clean-grade female SD rats were randomly divided into sham-operated group,model group,nylestriol group,quercetin high-dose group and quercetin low-dose group,10 cases in each group. The rats in sham-operated group were treated with sham-operation,while the rats in other 4 groups were treated with bilateral ovariectomy. One week after the modeling,the rats in sham-operated group and model group were intragastric administrated with normal saline,once a day,4 ml at a time; and the rats in nylestriol group were intragastric administrated with nilestriol（ 1. 5 mg / kg）,once a week; and the rats in quercetin high-dose group and quercetin low-dose group were intragastric administrated with quercetin（ 200 and 100 mg / kg,respectively）,once a day. Three months later,the serum osteocalcin levels were measured by using enzyme linked immunosorbent assays and the typeⅠcollagen protein levels of femoral metaphysis were measured by using Western Blot assays. Results： Some rats died from pulmonary infection in model group（ 1）,nilestriol（ 1）,quercetin high-dose group（ 1） and quercetin low-dose group（ 2） at the end of drug intervention. There was statistical difference in serum osteocalcin level between the 5 groups（ 2. 562 ＋ /- 0. 671,5. 763 ＋ /- 0. 612,2. 674 ＋ /- 1. 170,2. 722 ＋ /- 1. 063,3. 574 ＋ /-1. 914 ng / ml,F = 98. 527,P = 0. 000）. The serum osteocalcin levels were lower in sham-operated group,nilestriol group,quercetin high-dose group and quercetin low-dose group compared to model group（ P = 0. 000,P = 0. 000,P = 0. 000,P = 0. 005）. There was no statistical difference in the serum osteocalcin levels between quercetin high-dose group and nilestriol group（ P = 0. 361）. The serum osteocalcin level were higher in quercetin low-dose group compared to nilestriol group（ P = 0. 047）. There was no statistical difference in serum osteocalcin levels between quercetin low-dose group and quercetin high-dose group（ P = 0. 106）. There was statistical difference in typeⅠcollagen protein levels of femoral metaphysis between the 5 groups（ 2. 718 ＋ /- 0. 362,1. 492 ＋ /- 0. 228,2. 624 ＋ /- 0. 296,2. 497 ＋ /- 0. 274,1. 713 ＋ /- 0. 327,F = 10. 726,P = 0. 000）. The type Ⅰ collagen protein levels were higher in sham-operated group,nilestriol group and quercetin high-dose group compared to model group（ P = 0. 000,P = 0. 000,P = 0. 000）. There was no statistical difference in the typeⅠcollagen protein levels between quercetin low-dose group and model group（ P = 0. 056）. There was no statistical difference in the typeⅠcollagen protein levels between quercetin high-dose group and nilestriol group（ P = 0. 217）. The typeⅠcollagen protein levels were lower in quercetin low-dose group compared to quercetin high-dose group and nilestriol group（ P = 0. 000,P = 0. 000）. Conclusion： Quercetin has estrogen-like effects,which can effectively reduce serum osteocalcin levels and raise femur typeⅠcollagen protein levels in ovariectomized rats,and its effect is related to the dose.