五味子多糖对人脑胶质瘤U87细胞活力的影响及其机制

Effects of schisandra chinensis polysaccharide on cell activity of human brain glioma U87 cells

目的观察五味子多糖(CPSC)对人脑胶质瘤U87细胞活力的影响,并探讨其可能作用机制。方法取适量对数生长期人脑胶质瘤U87细胞,分为A、B、C组和对照组,A、B、C组分别加入终浓度为200、400、800μg/m L的CPSP培养,对照组加入等体积细胞培养液培养,分别于培养24、48、72 h时观察各组细胞活力;培养48 h时取各组培养液上清,检测半胱氨酸天冬氨酸蛋白酶3(Caspase-3)。结果培养24、48、72 h时,A组细胞存活率分别为1.17±0.04、1.09±0.05、1.39±0.05,B组分别为1.01±0.03、1.01±0.03、1.21±0.02,C组分别为0.90±0.02、0.76±0.02、0.98±0.03,对照组分别为1.28±0.03、1.16±0.03、1.45±0.01;培养72 h时,B组细胞存活率与对照组相比,P<0.05;培养24、48、72 h时,C组细胞存活率与对照组相比,P均<0.05。培养48 h时A、B、C组及对照组培养液上清Caspase-3蛋白的相对表达量分别为0.79±0.02、0.96±0.01、1.13±0.01、0.78±0.01,B、C组与对照组相比,P均<0.05。结论高浓度CPSC可抑制U87细胞的活力,其机制可能是与CPSC促进细胞Caspase-3表达有关。

Objective To observe the effects of schisandra chinensis polysaccharides（ CPSC） on the cell activity of human brain glioma U87 cells,and to explore its possible mechanism. Methods The human glioma U87 cells in the logarithmic growth phase were divided into groups A,B,C and control group. The cells in the groups A,B and C were added with the final concentrations of 200,400 and 800 μg / m L of CPSP to culture. The control group was treated with the equal volume of cell culture fluid. The cell viability was observed at 24,48 and 72 h after culture. At 48 h,the cysteine aspartic acid proteinase 3（ Caspase-3） in the nutrient solution supernatant was detected. Results At 24,48 and 72 h,the cell survival rates of group A were respectively 1. 17 ± 0. 04,1. 09 ± 0. 05 and 1. 39 ± 0. 05. The cell survival rates of group B were respectively 1. 01 ± 0. 03,1. 01 ± 0. 03 and 1. 21 ± 0. 02. The cell survival rates of group C were respectively 0. 90 ±0. 02,0. 76 ± 0. 02 and 0. 98 ± 0. 03. The cell survival rates of the control group were respectively 1. 28 ± 0. 03,1. 16 ±0. 03 and 1. 45 ± 0. 01. At 72 h,the cell survival rate of group B was statistically significant as compared with that of the control group（ P〈0. 05）. Significant difference was found in the survival rate between group C and control group at 24,48 and 72 h（ P〈0. 05）. The relative expression of Caspase-3 protein in cultural supernatant of groups A,B,C and control group was respectively 0. 79 ± 0. 02,0. 96 ± 0. 01,1. 13 ± 0. 01 and 0. 78 ± 0. 01,and significant difference was found between the groups B,C and the control group（ all P〈0. 05）. Conclusion High concentration of CPSC can inhibit the viability of U87 cells,and its mechanism may be that cpsc promote the Caspase-3 expression.