WT1在浆液性乳头状子宫内膜腺癌中的表达及临床意义

Expression of WT1 in serous endometrial carcinoma and its clinical significance

目的：探讨浆液性乳头状子宫内膜腺癌中 WT1蛋白的表达，观察 WT1的表达对患者预后生存的影响。方法选取71例浆液性乳头状子宫内膜腺癌癌标本，采用免疫组织化学方法对浆液性乳头状子宫内膜腺癌组织中的 WT1蛋白表达进行检测，观察 WT1的表达对患者预后生存的影响。结果31例浆液性乳头状子宫内膜腺癌标本 WT-1表达阳性，阳性表达率为43.7％。WT1蛋白在浆液性乳头状子宫内膜腺癌中表达与组织学分级、临床 FIGO 分期、肌层浸润程度、淋巴结转移、宫颈受累、宫外扩散方面比较差异未见统计学意义（P ﹥0.05）。WT1阴性组病例5年生存率为42.5％，而 WT1阳性组病例5年生存率为12.9％，两组比较差异有统计学意义（ P ﹤0.05）。结论 WT1基因表达与浆液性乳头状子宫内膜腺癌预后密切相关，可作为浆液性乳头状子宫内膜腺癌的新的治疗靶点和预后标志物。

Objective To investigate the expression of WT1 protein in papillary serous adeno-carcinoma of endometrium, and to observe its effect on the prognosis and expression of WT1. Methods Seventy-one cases of serous papillary adenocarcinoma of endometrium carcinoma,immunohis-tochemistry method was used to detect the expression of papillary serous endometrial carcinoma WT1 pro-tein,and observed the expression of WT1 effect on prognosis. Results Thirty-one cases of serous papil-lary adenocarcinoma of the endometrium specimens were WT-1 positive,the positive expression rate was 43. 7% . The difference of WT1 protein in papillary serous adenocarcinoma of endometrium expression and histological grade,clinical FIGO stage,myometrial invasion,lymph node metastasis,cervical in-volvement,ectopic proliferation had no statistically significant（P ﹥ 0. 05）. The 5 years survival rate of WT1 negative patients was 42. 5% ,and the 5 years survival rate of WT1 positive patients was 12. 9% , the difference was significant between the two groups（P ﹤ 0. 05）. Conclusions The expression of WT1 gene is closely correlated with papillary serous endometrial cancer prognosis,it can be used as targets for therapy and prognosis of serous papillary plaint endometrial adenocarcinoma marker.