肥胖对外周血白细胞胆固醇逆转运相关基因表达的影响

Effects of obesity on the expression of reverse cholesterol transport genes in peripheral blood leukocytes

目的 研究肥胖者外周血白细胞胆固醇逆转运相关基因表达的情况.方法 采用实时荧光定量PCR方法,测定肥胖组和对照组外周血白细胞胆固醇逆转运相关基因ABCA1、ABCG1和ApoA1 mRNA表达水平.结果 肥胖组ABCG1mRNA表达水平较对照组显著降低（0.536±0.271比6.243±1.511,P＜0.05）,ApoA1mRNA表达水平较对照组升高（0.385±0.188比0.206±0.062,P＜0.05）.单因素相关分析显示,ABCG1mRNA表达与体重指数BMI呈负相关（r=-0.403,P=0.012）.多元线性回归分析提示,ABCG1mRNA表达与体重指数BMI呈独立相关性（β=-0.488,P=0.004）.结论 肥胖者外周血白细胞ABCG1mRNA表达下调,且ABCG1mRNA与体重指数BMI呈负相关,这可能是肥胖导致动脉粥样硬化发生、发展的机制之一.

Objective To study the expressions of reverse cholesterol transport genes in peripheral blood leukocytes in obese patients. Methods The expressions of peripheral blood leukocytes ATP binding cassette transporter A1 （ABCA1）, ATP binding cassette transporter G1 （ABCG1） and apolipoprotein A1 （ApoA1） mRNA in obesity group and control group were measured by real-time quantitative PCR （RT-PCR）. Results Compared with control group, ABCG1 mRNA expression decreased （0.536±0.271 vs 6.243±1.511, P〈0.05） and ApoA1 mRNA expression increased （0.385±0.188 vs 0.206±0.062, P〈0.05）in obesity group. Single factor correlation analysis showed that the expression of ABCG1 mRNA was negatively correlated with body mass index （BMI）（r= -0.403,P=0.012）. Using multivariant linear regression analysis method, it was shown that the expression of ABCG1 mRNA was dependently associated with obesity （fl=-0.488,P=0.004）. Conclusion The expression of ABCG1 mRNA in peripheral blood leukocytes was decreased in obesity group and was negatively correlated with BMI, which may be one of the mechanisms that obesity results in atheroselerosis.