微酸性电解水对苹果连作土壤环境的影响

Effects of Slightly Acid Electrolyzed Oxidizing Water on Apple Replanted Soil Environment

以平邑甜茶幼苗(Malus hupehensis Rehd.)为试材,在盆栽条件下研究了连作土壤浇灌微酸性(pH 6.0)电解水对土壤环境和平邑甜茶幼苗生长的影响。结果表明,连作土壤中浇入微酸性电解水能够显著提高平邑甜茶幼苗的生物量,与连作土对照相比,幼苗根长度、根表面积、根体积和根系呼吸耗氧速率分别提高了47.03%、141.79%、101.31%和37.31%,连作土壤中真菌数量降幅达48.1%,细菌/真菌比值增加120%;应用T-RFLP技术分析了连作土壤真菌的群落结构,并采用多样性指数分析、聚类分析和主成分分析,发现微酸性电解水处理的土壤真菌群落具有最高的丰富度指数、多样性指数、均匀度指数和最低的优势度指数,而连作土壤的真菌群落具有最高的优势度指数和最低的丰富度指数、多样性指数、均匀度指数。微酸性电解水处理后,土壤真菌构成一个独立的群落结构,这些群落能够适应电解水处理后的土壤环境并成为环境的优势群落。微酸性电解水可减轻苹果连作障碍。

Effects of SAEW（Slightly Acid Electrolyzed Oxidizing Water,p H 6.0）on the replanted soil environment and Malus hupeheusis Rehd.seedlings biomass were investigated in pot experiments.Results showed that SAEW significantly enhanced Malus hupeheusis Rehd.seedling biomass.Compared with the control,SAEW increased the length of roots,surface area of roots,total roots volume and root activity by 47.03%,141.79%,101.31% and 37.31%,respectively.Compared with the control,SAEW decreased the numbers of fungi by 48.1%,and the ratio of bacteria to fungi increased by 120%.T-RFLP（Terminal Restriction Fragment Length Polymorphism）was applied in the analysis of soil fungal diversity.Based on the T-RFLP,diversity index analysis,cluster analysis and principal component analysis,the simpson index of SAEW on the replanted soil was improved,Shannon index,Pielou index and Margalef index were reduced significantly.However,the SAEW treatment soil expressed a reverse trend.SAEW on the replanted soil formed independent community structure,and these communities could adapt to their own specific soil environment and become the dominant population.SAEW could reduce apple replant disease.