玉屏风散对肺气虚证模型大鼠免疫功能的影响

Effects of Yupingfeng Powder on Immune Function of Rats with Lung-qi Deficiency Syndrome

目的:考察玉屏风散对肺气虚证模型大鼠免疫功能的影响。方法:将60只SD大鼠随机分为正常组、模型组、阳性组[人参北芪片0.6 g(生药)/kg]和玉屏风散高、中、低剂量组[24、12、6 g(生药)/kg],每组10只。除正常组外,其余各组大鼠均采用二氧化硫+风寒综合刺激法复制肺气虚证模型。造模成功后,各给药组大鼠ig相应药液,正常组和模型组大鼠ig等体积生理盐水,每天1次,连续13 d。给药结束后,测定大鼠血清中白细胞介素(IL)-3、IL-6水平及T淋巴细胞中CD_3^+、CD_4^+、CD_8^+比例,观察肺、支气管组织病理变化。结果:与正常组比较,模型组大鼠血清中IL-3、IL-6水平及T淋巴细胞中CD_8^+比例明显升高,T淋巴细胞中CD_3^+、CD_4^+比例及CD_4^+/CD_8^+比值降低(P<0.01);肺、支气管明显损伤并伴有炎症反应。与模型组比较,阳性组和玉屏风散高剂量组大鼠血清中IL-3水平明显降低;阳性组和玉屏风散高、中剂量组大鼠血清中IL-6水平、T淋巴细胞中CD_8^+比例明显降低,T淋巴细胞中CD_3^+比例明显升高;各给药组大鼠血清中CD_4^+比例、CD_4^+/CD_8^+比值明显升高(P<0.05或P<0.01);肺、支气管损伤及炎症反应程度减轻。结论:玉屏风散可增强肺气虚证模型大鼠的免疫功能。

OBJECTIVE：To study the effects of Yupingfeng powder on immune function of rats with lung-qi deficiency syndrome.METHODS：60 SD rats were randomly divided into normal group,model group,positive group[Renshenbeiqi tablet 0.6 g（crude drug）/kg]and Yupingfeng powder high-dose,medium-dose and low-dose groups[24,12,6 g（crude drug）/kg],with 10 rats in each group.Except for normal group,those groups were given SO2＋comprehensive cold stimulation to induce lung-qi deficiency syndrome model.After modeling,treatment groups were given relevant drug solutions intragastrically,and normal group and model group were given constant volume of normal saline intragastrically,once a day,for consecutive 13 d.After administration,serum levels of IL-3 and IL-6,the proportion of CD3＋,CD4＋,CD8＋ in T lymphocyte were determined,and pathological changes of lung and bronchus were observed.RESULTS：Compared with normal group,serum levels of IL-3 and IL-6 and the proportion of CD8＋ in T lymphocyte increased significantly in model group,while the proportions of CD3＋ and CD4＋ in T lymphocyte and the ratio of CD4＋/CD8＋ decreased（P〈0.01）;obvious lung and bronchus injury and inflammatory reaction were observed.Compared with model group,serum level of IL-3 decreased significantly in positive group and Yupingfeng powder high-dose group;serum level of IL-6 and the proportion of CD8＋ in T lymphocyte decreased significantly in positive group and Yupingfeng powder high-dose and medium-dose groups,while the proportion of CD3＋ in T lymphocyte increased significantly;the proportion of CD4＋and the ratio of CD4＋/CD8＋ increased significantly in treatment groups（P〈0.05 or P〈0.01）;lung and bronchial injury,and inflammation reaction were relieved.CONCLUSIONS：Yupingfeng powder can enhance immune function of rats with lung-qi deficiency syndrome.