柏薏灌肠液的提取工艺研究

Optimization of Extraction Technology for Baiyi Rectal Solution

目的:优化柏薏灌肠液的提取工艺。方法:采用星点设计-响应面法。以盐酸小檗碱、阿魏酸和干膏率的总评归一值(OD)为评价指标,考察加水量和提取时间对提取工艺的影响;同时进行验证试验。结果:最优提取工艺为第1次加11倍水煎煮70 min,第2次加5倍水煎煮30 min。验证试验中第1次提取的OD值为0.940 0,与预测值偏差-2.07%;第2次提取的OD值为0.851 8,与预测值偏差-2.41%。结论:柏薏灌肠液的提取工艺合理可行,适用于工业化生产。

OBJECTIVE：To optimize the extraction technology of Baiyi rectal solution. METHODS：The effects of water amount and extraction time on extraction technology were investigated by central composite design-response surface method using OD value of berberine hydrochloride and ferulic acid and the extract yield as index. Validation test was also conducted. RESULTS：The optimal extraction technology was as 11-fold water,decocting 70 min for the first time;5-fold water,decocting 30 min for the second time. In validation test,OD value of first extraction was 0.940 0,and the bias between observed and predicted values was-2.07%;that of second extraction was 0.851 8,and the bias was-2.41%. CONCLUSIONS：The extraction technology of Baiyi rectal solution is reasonable,feasible and suitable for industrial production.