强直性脊柱炎患者外周血CD4+、CD8+ T细胞TCR BV CDR3多态性分析

TCR BV CDR3 polymorphism study on CD4+,CD8+ T cells lineage in peripheral blood of ankylosing spondylitis patients

为探讨AS患者外周血CD4＋、CD8＋T细胞TCR BV CDR3谱型多样性特点,为AS免疫发病机制及治疗提供基础依据。应用RT-PCR方法分别扩增AS患者CD4＋、CD8＋T细胞TCR BV 26个亚家族CDR3区,采用免疫指纹技术进行TCR BV各家族基因扫描和谱型分析。结果显示,正常健康人CD4＋、CD8＋TCR BV绝大多数亚家族谱型呈现正态分布（或高斯）分布;AS患者CD4＋T细胞绝大多数TCR BV家族呈现与健康对照相似的正态分布,仅少数家族呈不规则异常峰型或偏峰,极少家族出现寡克隆趋势和单克隆,不同患者的CD4＋TCR BV家族异常峰型率在4%（1/26）~27%（7/26）;AS患者CD8＋T细胞,大多数BV家族呈现非正态异常峰型,而少部分家族呈现正态分布的钟型峰图,不同患者CD8＋TCR BV家族异常分型率在27%（7/26）~77%（20/26）,呈现寡克隆趋势,寡克隆和单克隆峰型的家族数量明显多于CD4＋T细胞。由此可知,T细胞在AS的免疫发病机理中扮演重要角色,且提示CD8＋T细胞在AS发病机制中起主导作用。

To study CD4＋, CD8＋T cells lineage polymorphism of TCR BV CDR3 in peripheral blood of ankylosing spondylitis patients, so as to provide foundation basis to immune pathogenesis research in ankylosing spondylitis. CD4＋, CD8＋ T cells TCR BV 26 subfamily CDR3 in ankylosing spondylitis patients PBMC were amplified by RT- PCR method, then TCR BV CDR3 lineages polymorphism were analysed by immunization scanning spectrum. Most spectral type of CD4＋, CD8＋ T cells TCR BV CDR3 in normal controls showed Gauss distribution（or Gauss）; Most CD4＋T cells TCR BV CDR3 scanning spectrum of ankylosing spondylitis patients showed Gauss distribution（or Gauss） were similar to the normal controls, a few CD4＋ T cells TCR BV CDR3 showed skewing peak or a irregular abnormal peak, and a rare CD4＋ T cells TCR BV CDR3 showed oligoclonal trend and monoclonal, the rate of different subsets of abnormal peaks was between 4%（1/26） and 27%（7/26） in different patients;Most spectral type of CD8＋TCR BV CDR3 showed abnormal distribution peak and a few of it showed Gauss distribution, the rate of different subsets of abnormal peaks was between 27%（7/26）and 77%（20/26） in different patients, the number of abnormal peak was more than CD4＋ T cells TCR BV CDR3,including oligoclonal/oligoclonal trend and monoclonal.The study not only further indicates that T cells play an essential role in immune pathogenesis of ankylosing spondylitis, but also suggests CD8＋T cells play a dominant role in immune pathogenesis of ankylosing spondylitis.