UPLC指纹图谱结合化学计量学的白花蛇舌草真伪鉴别研究

Identification of Oldenlandia diffusa by UPLC Fingerprint Coupled with Chemometrics Methods

目的:建立白花蛇舌草及其伪品水线草的UPLC指纹图谱,结合化学计量学,进行真伪鉴别。方法:建立白花蛇舌草和水线草的UPLC指纹图谱,对主要色谱峰进行化学指认及快速检识,利用两者化学成分差异结合化学计量学分析,开展真伪鉴别。结果:分别建立了两者UPLC指纹图谱方法并评价了多批次的相似度;白花蛇舌草22个共有峰中的10个、水线草16个共有峰中的5个及4个非共有峰得到了指认或检识;主成分分析(PCA)可将真品、伪品分为两组;而偏最小二乘法分析(OPLS-DA)显示车叶草酸甲酯等5个色谱峰是分组的主要原因;白花蛇舌草中的(E)-6-O-阿魏酰鸡屎藤次苷甲酯、(Z)-6-O-香豆酰鸡屎藤次苷甲酯,水线草中的槲皮素-3-O-桑布双糖苷可作为两者的3个特征差异峰用于白花蛇舌草真伪鉴别。结论:将UPLC指纹图谱与化学计量学方法相结合,可用于白花蛇舌草真伪鉴别,从而为提高白花蛇舌草整体质量控制方法提供参考。

Objective： To establish UPLC fingerprints for Oldenlandia diffusa and Oldenlandia corymbosa,coupled with chemometrics methods,so as to identify Oldenlandia diffusa. Methods： The fingerprints of Oldenlandia diffusa and Oldenlandia corymbosa were established,main peaks were designed and identified. The differences of chemical compositions coupled with chemometrics methods were used to identify Oldenlandia diffusa. Results： The fingerprints of Oldenlandia diffusa and Oldenlandia corymbosa were established and the similarities were evaluated. 10 of 22 common peaks in Oldenlandia diffusa,5 of 16 common peaks and 4 private peaks in Oldenlandia corymbosa were signed and identified. Oldenlandia diffusa and Oldenlandia corymbosa could be classified into two clusters by principal components analysis（ PCA）. OPLS-DA indicated that five chromatographic peaks such as asperulosidic acid methyl ester were the main reason for grouping. The three special peaks：（ E）-6-O-feruloyl scandoside methy1 ester,（ Z）-6-O-p-coumaroyl scandoside methyl ester and quercetin 3-O-sambubioside in Oldenlandia diffusa and Oldenlandia corymbosa could be used to identify Oldenlandia diffusa. Conclusion： The establishment of UPLC fingerprint coupled with chemometrics methods can be used to identify Oldenlandia diffusa,so as to provide a more comprehensive reference for the quality control of herbs.