柱前衍生化高效液相色谱法测定鳖甲生、制品中氨基酸的含量

Determination of the Amino Acids in the Raw and Processed Products of Turtle Shell by HPLC Combining Pre-column Derivatization

目的：建立柱前衍生化高效液相色谱法分析鳖甲生、制品中氨基酸的组成和含量。方法：采用酸水解法制备鳖甲生、制品的氨基酸样品溶液,以邻苯二甲醛（OPA）和9-芴甲基氯甲酸酯（FMOC）为柱前衍生化试剂,采用C18色谱柱,梯度洗脱。结果：18种氨基酸在38 min内得到较好分离,在一定浓度范围内线性关系良好（r≥0.9991）,鳖甲生、制品中检出的16种氨基酸的平均加样回收率在90.43%~110.69%之间,RSD在0.76%~5.89%之间（n=6）。结论：该方法分离效果较好,可用于测定鳖甲生、制品的氨基酸含量。

Objective： To establish the pre-column derivatization combining HPLC to determine the contents of the amino acids of the raw and processed products of Turtle shell. Methods： The samples were prepared by the acidic hydrolysis method,derivatized by OPA and FMOC,and analyzed by C18 column with gradient elution. Results： 18 kinds of amino acids were separated within 38 min,which showed a good linear relationship（ r ≥0. 9991）. The average recoveries of 16 amino acids in the samples were between 90. 43% ~110. 69%,and the RSD were between 0. 76% ~ 5. 89%（ n = 6）. Conclusion： This method can be used to determine the contents of the amino acids in the raw and processed products of Turtle shell.