正交试验优选薏苡附子败酱散提取工艺

Optimization of Extraction Technology of Yiyi Fuzi Baijiang Powder by Orthogonal Design

目的:优选薏苡附子败酱散的提取工艺。方法:采用正交试验法,以薏苡仁油的提取率为评价指标,优选薏苡仁的醇提工艺;以原儿茶酸、绿原酸、苯甲酰新乌头原碱的转移率为评价指标,优选附子和败酱草的水提工艺。结果:薏苡仁最佳提取工艺为:加4倍量无水乙醇,超声提取3次,每次20 min。附子和败酱草最佳提取工艺为:提取2次,即第1次附子加10倍量水煎煮1.0 h,然后加入败酱草,共同煎煮45 min;第2次药渣加8倍量水,再煎煮30 min。结论:优选的提取工艺稳定、可行,为薏苡附子败酱散的制剂开发提供了理论依据。

Objective： To optimize the optimum extraction technology of Yiyi Fuzi Baijiang powder. Methods： Orthogonal design was used,the extraction rate of Coicis Semen oil was used to determine the ethanol extraction technology of Coicis Semen,the extraction rate of protocatechuic acid,chlorogenic acid and benzoylmesaconine were used to determine the water extraction technology of Aconiti Lateralis Radix Praeparata and Herba Patriniae. Results： The optimum extraction technology of Coicis Semen was obtained,alcohol was consumed as four times of the herb amount,and ultrasonic extracted three times with 20 minutes each time; the optimum extraction technology of Aconiti Lateralis Radix Praeparata and Herba Patriniae was obtained,water was consumed as ten times of the herb amount,and extracted for 1. 0 h for Aconiti Lateralis Radix Praeparata and then Herba Patriniae was added and extracted for 45 minutes for the first time; in the second time,water was consumed as 8 times of the herb amount,and extracted for 30 minutes for dregs of herbs. Conclusion： The optimum extraction technology is stable and feasible,in which can give a reference to the preparation research of Yiyi Fuzi Baijiang powder.