玄参及其拆分组分对异丙肾上腺素致大鼠心室重构的影响

Effects of Scrophulariae Radix and Split Component on Isoproterenol-Induced Ventricular Remodeling in Rat

目的:研究玄参及其拆分组分对异丙肾上腺素(ISO)诱发大鼠实验性心室重构的药理作用及机制。方法:采用大鼠背部皮下注射ISO,连续2 d以递减剂量10、5 mg/kg进行,3 mg/kg的剂量维持注射7 d,制备心室重构模型。经药干预21 d后,检测大鼠左心室(LVMI)及全心室质量指数(HMI);酶联免疫法检测血清心房利钠肽(ANP)、内皮素-1(ET-1)及心肌组织血管紧张素Ⅱ(AngⅡ)含量,同时进行心肌组织病理学观察。结果:玄参全成分、小极性环烯醚萜苷组分可改善模型大鼠LVMI、HMI,降低血清ANP、ET-1及心肌组织AgnⅡ含量;多糖组分仅能降低AngⅡ、ET-1的含量。结论:玄参抑制心室重构的药理作用与小极性环烯醚萜组分和多糖组分有关。其作用机制可能与调节过度激活的神经体液因子,抑制ANP、ET-1、AngⅡ的释放有关。

Objective： To explore the effects and mechanism of Scrophulariae Radix and split component on experimental ventricular remodeling. Methods： The rats were injected subcutaneously by 10,5 mg / kg ISO for 2 d and then 3 mg / kg ISO as maintenance dose for 7 d to build the the ventricular remodeling. After 21 days＇ treatment,the left ventricular mass index（ LVMI） and heart mass index（ HMI） were measured. The content of atrial natriuretic peptide（ ANP）,endothelin-1（ ET-1） and angiotensinⅡ（ Ang II） were determined by enzyme linked immunosorbent assay（ ELISA）,and the pathological changes of myocardial tissue were also observed. Results： LVMI,HMI were improved by total composition components and small polar iridoid glycosides components,and the content of ANP,ET-1 and AngⅡwas decreased remarkably; The polysaccharide components could only decline the content of Ang Ⅱ and ET-1. Conclusion： The pharmacological effects of Scrophulariae Radix inhibit ventricular remodeling may be related to the small polar iridoid glycosides components and the polysaccharide components. And the mechanism may be related to regulating the over expression neurohumor factors and inhibiting the release of ANP and ET-1,Ang Ⅱ.