山楂叶不同采收期多种有效成分含量的比较

Comparison of the active ingredient contents in hawthorn leaves samples of different harvests

目的探索山楂叶最佳采收期。方法 HPLC条件:金丝桃苷检测采用Shim-pack C18(150mm×4.6mm,5μm);乙腈-甲醇-四氢呋喃-5 mL·L^(-1)醋酸溶液(1∶1∶19.4∶78.6)为流动相,检测波长为363nm。牡荆素鼠李糖苷检测采用Diamonsil C18柱(250mm×4.6mm,5μm);四氢呋喃-甲醇-乙腈-乙酸-水(38∶3∶3∶4∶152)为流动相;检测波长为330nm。总黄酮检测利用可见分光光度法,以芦丁对照品为对照,NaNO2-Al(NO3)3-NaOH体系作显色剂,在500nm波长处测定总黄酮含量。结果金丝桃苷进样量在0.080 5~0.402 4μg范围内,质量浓度与峰面积线性关系良好(r=0.999 3);牡荆素鼠李糖苷进样量在0.418~2.090μg范围内,质量浓度与峰面积线性关系良好(r=0.999 1)。结论 5月采收的山楂叶中牡荆素鼠李糖苷含量最高;8~11月采收的山楂叶中金丝桃苷及总黄酮的含量较高。

Objective To explore the suitable time to harvest hawthorn leaves.Methods HPLC was used and the conditions were as follows：Shim-pack C_（18）（150mm×4.6mm,5μm）column with the mobile phase consisted of acetonitrile-methanol-tetrahydrofuran-5 mL·L^（-1）acetic acid（1∶1∶19.4∶78.6）in the separation of hyperin,and the detection wavelength was set at 363 nm.The separation of vitexin-2-O-rhamnoside was performed on the Diamonsil C18（250mm×4.6mm,5μm）column with the mobile phase consisted of tetrahydrofuran,acetonitrile,methanol,acetic acid and water（in a ratio of 38∶3∶3∶4∶152）.The detection wavelength was set at 330 nm.The content of total flavone in hawthorn leaves was determined by spectrophotometry.To compare with the rutin reference substance,NaNO_2-Al（NO_3）_3-NaOH was used as the color development reagent.The detection wavelength was set at 500 nm.Results The linear relationship between area and amount was noted for hyperin with the amount of 0.080 5-0.402 4μg（r=0.999 3）;The linear relationship between area and amount was noted for vitexin-2-O-rhamnoside with the amount of 0.418-2.090μg（r=0.999 1）.Conclusion The content of vitexin-2-O-rhamnoside in hawthorn leaves which were harvested in May was the highest;The contents of hyperin and total flavone in hawthorn leaves which were harvested from August to November were relatively high.