健脾清化中药复方对慢性萎缩性胃炎大鼠TLR4/NF-κB/COX-2信号通路的影响

Effects of Jianpi Qinghua TCM compound on the TLR4/NF-κB/COX-2 signaling pathway in rats with chronic atrophic gastritis

[目的]探讨健脾清化中药复方对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠TLR4/NF-κB/COX-2信号通路的影响。[方法]wistar大鼠随机分为空白组、造模组。造模组大鼠前12周利用20mmol/L去氧胆酸钠溶液灌胃(2ml/次/d)联合60%乙醇空腹灌胃(每次2ml,2次/周)及0.5g/L氨水自由饮用,建立CAG大鼠模型。确认造模成功后,将造模组大鼠随机分为模型组、中药低剂量组、中药高剂量组各8只;将空白组大鼠随机分为空白对照组和空白中药组各8只。中药低剂量、高剂量组、空白中药组分别按照1.5ml/kg、6ml/kg、6ml/kg的健脾清化复方原液剂量并予生理盐水补齐至4ml灌胃,空白对照组及CAG模型组给予生理盐水4ml灌胃,均1次/d,给药30d。观察各组大鼠的胃组织病理组织学变化,以及对TLR4、NF-κB、COX-2、MYD88mRNA表达量的影响。[结果]中药干预组胃黏膜萎缩情况明显好转,与模型组比较显著差异(P<0.01),以中药高剂量组疗效更为显著。与空白组比较,模型组、中药高、低剂量组TLR4、NF-κB、COX-2、MYD88mRNA水平均明显增高(P<0.01)。同模型组比较,中药高、低剂量组TLR4、NF-κB、COX-2、MYD88mRNA水平有下调(P<0.01),特别以中药高剂量组表现明显。[结论]健脾清化中药复方对CAG大鼠胃黏膜组织病理有明显改善作用,并可有效阻断TLR4/NF-κB/COX-2信号通路的持续活化。

[Objective]To observe the effects of Jianpi Qinghua TCM compound on the activity of the TLR4,NF-κB,COX-2and MYD88 signaling pathway in rats with chronic atrophic gastritis（CAG）.[Methods]CAG rat models were established by intragastric administration with deoxycholic acid sodium salt（2ml once a day）with concentration of 20mmol/L,60% ethanol（2ml twice a week）lavaging on an empty stomach and giving 0.5g/L aqueous ammonia to drink freely for 12 weeks.CAG models were divided into 3groups at random：model group（MG）,low-dose group of Chinese medicine（LDG）and high-dose group of Chinese medicine（HDG）,8rats per group.Normal rats were randomly divided into blank control group（BCG）and blank control group of Chinese medicine（BCGCM）,8rats per group.1.5ml/kg,6ml/kg and 6ml/kg of Jianpi Qinghua TCM compound were given to the LDG,HDG and BCGCM with the dose and up to 4ml with normal saline,once a day for 30 days.Respectively,animals in BCG and MG were only given 4ml normal saline.Pathological changes of the rats stomach tissue were observed and the expression of TLR4,NF-κB,COX-2and MYD88 mRNA was measured respectively.[Results]The gastric mucosal atrophy in the groups treated with Jianpi Qinghua TCM compound improved markedly,showing significant difference as compared with that in the MG（P〈0.01）.The improvement in the HDG was the best.Besides,compared with the BCG,the expression of TLR4,NF-κB,COX-2,MYD88 mRNA increased especially in the MG,LDG and HDG,showing statistical significance（P〈0.01）.Compared with the MG,the expression of TLR4,NF-κB,COX-2and MYD88 mRNA reduced in the LDG and HDG,showing statistical significance（P〈0.01）.[Conclusion]Jianpi Qinghua TCM compound had siginificant effect on improving the pathological changes of gastric mucosa.Besides,it could inhibit the continuous activation of TLR4,NF-κB,COX-2signal pathway.