SPE-HPLC法检测牛肉及牛奶中阿维菌素残留

Solid-phase extraction and high performance liquid chromatographic detection of avermectin residues in beef and milk

为了建立一种可检测牛肉及牛奶中阿维菌素残留含量的方法，试验以流动相甲醇：水=90：10，检测波长为245nm，流速为0．8mL／min为检测条件的高效液相色谱法测定其含量。结果表明：17min内可将阿维菌素完全分离，线性范围为0．3～5．0μg／L，相关系数为0．9997，牛肉和牛奶中阿维菌素平均回收率分别为91．20％-100．32％、89．93％-99．15％，相对标准偏差分别小于7％、4％。该方法简便、灵敏、准确，适用于牛肉及牛奶中阿维菌素残留量的测定，并能满足国内和国外检测的要求。

To establish an effective method for determining the avermectin residues in beef and milk, the content of avermectin residues was de- termined by high performance liquid chromatography （HPLC） in the test. The mobile phase was V （methanol）：V （water） =90：10,245 nm was chose as the detection wave - length, and the flow rate was 0. 8 mL/min. The results showed that avermectin could be completely separated within 17 min, and the linear range was 0.3 to 5.0μg/L and the correlation coefficient was 0. 999 7. The average recoveries of avermectin in beef and milk were 91.20% to 100.32% and 89.93% to 99.15%, respectively. The relative standard deviations were less than 7% and 4%, respectively. The results indicate that the method is simple, sensitive and accurate, and it is suitable for determining avermectin residues in beef and milk, and can meet domestic and foreign requirements for detection.