缺血后处理对梗阻性黄疸大鼠肾缺血再灌注损伤IL-6及ATP的影响

Effect of ischemic postconditioning on IL-6 and ATP level during renal ischemia-reperfusion injury in obstructive jaundice rats

目的:探讨缺血后处理对梗阻性黄疸大鼠模型肾缺血再灌注损伤白细胞介素-6(interleukin-6,IL-6)、ATP的影响。方法 :SD大鼠100只,随机分为5组:正常对照组(Contrrol-Sham组)、正常肾缺血再灌注组(Control-I/R组),梗阻性黄疸组(OJ-Sham组)、梗阻性黄疸+肾缺血再灌注组(OJ-I/R组),梗阻性黄疸+肾缺血再灌注+缺血后处理组(OJ-I/R+IPO组),每组20例。根据缺血再灌注时间点,各组又分为4个亚组(n=5),分别以再灌注0、1、3、6 h为时间点,记为T_0、T_1、T_2、T_3。在胆道梗阻1周后,各组大鼠在常温下分别于T_0、T_1、T_2、T_34个时间点自大鼠下腔静脉采血,分离血清作检测用。处死大鼠并切除左肾,然后石蜡包埋行病理切片,并测定血清中IL-6及肾组织中ATP的含量。结果:与OJ-I/R组比较,OJ-I/R-IPO组从T_1开始,肾功能指标明显降低(P<0.05),BUN、Cr均明显降低(P<0.05),IL-6降低,ATP升高(P<0.05)。结论:缺血后处理可以使OJ大鼠的肾缺血再灌注损伤得到减轻,其机制可能与减轻炎症反应,减少能量代谢有关。

Objective To investigate the apoptosis effect of ischemic postconditioning on levels of IL-6and ATP during renal ischemia / reperfusion injury in obstructive jaundice rats. Methods One hundred SD rats were randomly divided into 5 groups ： the Sham control group, I / R group, OJ-Sham group, OJ-I / R group, and OJ- I / R＋IPO group, with 20 cases in each group. According to the time after ischemia / reperfusion, each group was divided into four subgroups（n = 5）, with reperfusion time of 0 h, 1 h, 3 h, 6 h, respectively. After 1week of biliary obstruction, rats were sacrificed at 0 h, 1 h, 3 h, 6 h post-I / R, and the left kidneys were taken, renal tissue was used for determination of the levels of interleukin-6（IL-6） and adenosinetriphosphate（ATP）. Results Compared with the OJ-I / R group, the serum level of BUN,Cr decreased significantly in the OJ-I / R-IPO group（P〈0.05）. Conclusion Ischemic postconditioning can reduce the injury of kidney ischemia-reperfusion in OJ rais, which may be related to the reduced inflammation reaction and the energy metabolism in the kidney.