摘要
为检验猪圆环病毒2型(PCV-2)重组多肽抗原对猪的免疫保护效果,以表达的重组大分子多肽作为抗原,断奶仔猪为实验动物,设置多肽疫苗组、常规市售Cap蛋白亚单位疫苗组和不同对照组进行免疫。用间接酶联免疫吸附法(ELISA)检测血清特异抗体(IgG),当抗体达到一定水平时进行攻毒试验,对试验各组的临床症状、体征变化及病毒血症进行观察和动态检测,对PCV-2易感组织进行病毒检测及组织病理学检查,最终评估该多肽疫苗的免疫效果及保护率。结果显示:表达的多肽疫苗能够快速产生体液免疫应答,具有显著的免疫效果;攻毒后采集的各项试验数据也表明该多肽疫苗的免疫保护效果理想。
In order to test the immune protection of recombinant polypeptide antigen of porcine cir- covirus type 2 （PCV-2） ,the recombinant polypeptide was selected as antigen,weaned pigs were se- lected as experimental animals,were divided into protein subunit vaccine group,Cap subunit com- mon sale vaccine group, and conventional set polypeptide vaccine. Indirect enzyme linked immu- nosorbent assay （ELISA） was used to detect the serum specific antibody （IgG） ,when the antibody reached a certain level, then the toxicity test was carried out, to dynamically clinical symptoms, viremia pathological findings of the susceptible tissues,and evaluate the immune effect of the poly- peptide vaccine. The results showed that the polypeptide vaccine can improve the humoral immune response, has good immune protection against PCV.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2016年第8期1278-1281,共4页
Chinese Journal of Veterinary Science
基金
广东省科技计划资助项目(2012A020603026)
