布鲁菌疫苗株S2全基因组测序及功能分析

Whole genome sequence of Brucellavaccine strain S2 and functional analysis

为找出布鲁菌疫苗株S2基因组分泌性蛋白、参与生物学途径基因、细胞组件基因、分子功能基因、毒力相关因子及耐药基因,对布鲁菌疫苗株S2全基因组测序并进行Ⅲ型分泌系统效应蛋白预测、GO注释、VFDB注释、ARDB注释。全基因组测序结果表明,S2基因组大小约为3.3 Mb,杂合度和重复度较低,GC含量分布未出现异常。通过Ⅲ型分泌系统效应蛋白预测未找出S2分泌性蛋白。通过GO数据库注释,发现参与生物学途径基因有3 823个、细胞组件基因有1 949个、分子功能基因有2 585个。通过VFDB数据库注释,找出S2基因组有79个毒力相关基因,其中最大基因为8 604bp,最小基因为180bp。通过ARDB数据库注释,找出S2基因组有13个耐药基因,最大基因为3 225bp,最小基因为333bp;其中功能注释的有GL002835基因,是枯草杆菌肽类基因,此基因耐受抗微生物类药物。本研究为进一步了解布鲁菌S2基因组基本功能奠定了基础。

In order to find the genome sequence of the secreted protein, biological process genes, molecular function genes, cellular component genes, virulence factors and antibiotic resistance genes of Brucella vaccine strain $2, the whole genome sequenceing of Brucella vaccine strain $2 was analyzed,and the type Ⅲ secretion system effectors prediction, GO terms, ARDB annotation and VFDB annotation were also made. The genome of Brucella vaccine strain S2 was 3.3 Mb and the degree of heterozygosity and repeatability was low, there was no abnormal in the GC content distribution. The secreted protein of strain $2 could not be found through the prediction of the type Ⅲ secretion system. Three thousand eight hundred and twenty three genes involved in biological process,1 949 and 2 585 genes associated with molecular function and cellular component were found respectively by the GO terms annotation. The analysis of virulence factor database（VFDB） showed that the $2 genome had 79 virulence-associated genes, the biggest gene was 8 604 bp,and the smallest one was 180 bp. Examination of antibiotic resistance genes database（ARDB） revealed that the 82 genome had 13 drug resistance genes,the largest gene was 3 225 bp,the smallest one was 333 bp. Among of which, GL002835 gene was fuctional annotated and was bacitracin, which had resist against the antimicrobial and antibacterial drugs. The research laid a foundation for fur- ther research on the function of the Brucella vaccine strain S2 genome.