犬同种异体半月板的移植

Investigation on meniscus allograft transplantation in dogs

为了探索犬半月板损伤的有效治疗方法,本研究建立了犬半月板缺损模型,并采用同种异体半月板全移植和半移植2种方式修复半月板缺损。术后第5周末,所有移植犬站立、行走和上台阶均趋于正常,但不移植的半月板缺损对照犬行走时仍有轻度跛行,上台阶困难。术后第6周膝关节功能Lysholm评分,全移植组和半移植组与正常对照组差异不显著,不移植组与对照组差异显著。肉眼检查见供体半月板在受体内生长良好。半移植组供体半月板的色泽和弹性更接近正常半月板。组织学观察,供体半月板和受体关节囊、供体半月板和保留的受体半月板接合良好,接合处组织生长旺盛。胫骨平台软骨Mankins评分,全移植组和半移植组与对照组差异不显著,不移植组与对照组差异显著。本研究结果表明同种异体半月板移植可有效保护胫骨平台软骨、改善膝关节功能,完全可以用于犬半月板损伤的治疗。

To explore effective treatment for the meniscus injury^meniscus injury models in dogs was made in this study, and either whole meniscus allograft transplantation or half meniscus al- lograft transplantation were used to repaire the meniscus defect. Five weeks after surgery, all transplanted dogs tended to stand,walk and climb stairs as normal, while the control dogs without meniscus walked with a slight limp and climbed stairs difficultly. Lysholm scores of knee joint function after surgery six weeks showed that there was not significant difference between the whole meniscus transplantation group and the half meniscus transplantation group with the normal control group,however,there was a significant difference between the group without transplanta- tion and the normal control group. Gross inspection showed that the donor meniscus grew well in the receptor dogs. The color and elasticity of donor meniscus were similar to those of the normal meniscus in the half meniscus transplantation group. Histological observation revealed that, the donor meniscus and the receptor＇s joint capsule as well as the donor meniscus and the receptor＇s re- served meniscus connected well,and the tissue was in a good condition in the junction. Mankin＇s scores of the tibial plateau cartilage suggested that there were not significant difference between the whole meniscus transplantation group and the half meniscus transplantation group with the normal control group, in contrary, there was significant differences between the group without transplantation and the control group. The research i＇evealed that meniscus allograft transplanta- tion could both ＇protect the tibial cartilage and improve the knee joint function effectively, indicating that it is a good way to treat meniscus iniury.