HPLC法同时测定桑菊止咳合剂中绿原酸、连翘苷、黄芩苷

Simultaneous Determination of Chlorogenic Acid,Forsythin,and Baicalin in Sangjuzhike Mixture by Dual-wavelength HPLC

目的建立同时测定桑菊止咳合剂中绿原酸、连翘苷和黄芩苷的HPLC方法。方法色谱柱为Agilent ZORBAX SB-C_(18)柱(250mm×4.6mm,5μm);流动相为甲醇-0.2%磷酸溶液,线性梯度洗脱,体积流量为1.0m L·min^(-1),检测波长为324、280nm,柱温35℃。结果绿原酸、连翘苷、黄芩苷的线性范围分别为0.0021~0.0471μg、0.0064~0.1269μg、0.03852~0.7703μg,平均加样回收率分别为97.8%、96.4%、99.3%。结论该方法简便、快速、准确,可作为该制剂的质量控制方法。

OBJECTIVE To establish an HPLC method for simultaneously determining chlorogenic acid , for-sythin,and baicalin in Sangjuzhike mixture .METHODS HPLC assay was carried out using an Agilent ZORBAX SB-C18 column with columntemperature at 35℃;The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL· min^-1;And the detection wavelengths were 324 and 280 nm.RESULTS The linear ranges of chlorogenic acid ,forsythin,and baicalin were 0.0021-0.0471μg,0.0064~0.1269μg and 0.03852~0.7703μg respectively ,and the average recoveries of three components were 97.8%,96.4%and 99.3%respectively.CONCLUSION The method is simple ,quick and accurate ,and may be used for the quality control of the mixture.