瑞香素对大鼠重症急性胰腺炎促炎因子的影响及意义

Effect and Significance of Daphnetin on Pro-inflammation Cytokines for Severe Acute Pancreatitis in Rats

目的:研究瑞香素对大鼠重症急性胰腺炎(SAP)血清中肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量的影响,探讨瑞香素对大鼠SAP的保护作用。方法:用5%牛磺胆酸钠1ml/kg经胆胰管逆行注射建立SAP模型。分为四组:假手术组(SO组)、药物对照组(SO-D组)、模型组(SAP组)和干预组(SAP-D组)。造模后分别在3,6,12h处死大鼠,对胰腺组织HE染色后观察病理损伤情况,用ELISA法测定血清中TNF-α和IL-1β的水平。结果:胰腺组织病理学评分,以及血清促炎细胞因子TNF-α和IL-1β水平,SAP-D组均较SAP组明显降低(P<0.05),SO组与SO-D组之间无明显差别(P>0.05)。结论:瑞香素具有较强抗炎活性,对大鼠SAP具有明显保护作用。

Objective：To study the effect of daphnetin on pro-inflammation cytokines for severe acute pancreatitis（SAP） in rats, and examine whether daphnetin has a protective effect on SAP. Meth- ods- The SAP rat model was induced by retrograde infusion of 5G sodium taurocholate （1 ml/kg） into the bile-pancreatic duct. The severity of pancreatitis was evaluated by determina- tion of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） levels, and hfstopathology grading. The levels of TNF-α and IL-β in the serum were measured by ELISA. Results. Daphne- tin-treated SAP rat group （SAP-D） exhibited a lower pathological score of pancreas as compared with SAP group （SAP） （P〈0.05）. Further analyses demonstrated that SAP-D group had lower levels of serum pro-inflammatory cytokines including TNF-α and IL-1β（P〈0.05） in 3, 6 and 12 h after the infusion of sodium taurocholate compared with SAP group. Conclusion： Daphnetin per- forms a protective function in the SAP rat model through the inhibition of pro-inflammatory fac- tors, thus providing a new way for the therapy of acute pancreatitis.