莲蓬醇提物中槲皮素的鉴定及生物活性

Identification of quercetin in ethanol extract of lotus seed pod and its biological activity

采用UV、TLC、IR、HPLC等方法鉴定莲蓬的80%乙醇提取物中的槲皮素,并研究其生物活性。结果表明:莲蓬醇提物中含有槲皮素成分;以VC和茶多酚作阳性对照,当莲蓬醇提物中槲皮素浓度达到8.658 3 mg/mL时,对·OH、O_2^-·、DPPH、ABTS+的清除率分别达到92.02%,98.54%,93.12%,91.32%;莲蓬醇提物对大肠杆菌、酵母菌和沙门氏菌的最低抑菌浓度均为0.8mg/mL(以槲皮素计),对金黄色葡萄球菌的最低抑菌浓度为0.6mg/mL(以槲皮素计),但对霉菌无明显抑制作用。莲蓬醇提物具有抗氧化性能和抑菌性能,且与槲皮素浓度呈正相关(R>0.980 0,P<0.05)。

The quercetin in the 80% ethanol extract of lotus seed pod was identified with UV,TLC,IR and HPLC,and its biology activity was also studied.Results：That the alcohol extract of lotus seed pod contained quercetin;Taking the VC and tea polyphenol as positive control,the·OH,O_2^-·,DPPH and ABTS＋scavenging activity of 92.02%,98.54%,93.12% and 91.32%,respectively,when the concentration of quercetin in the 80% ethanol extract of lotus seed pod reached 8.6583mg/mL;The MIC of the ethanol extract of lotus seed pod to escherichia coli,yeast and Salmonella was 0.8 mg/mL（quercetin concentration）,to staphylococcus aureus was 0.6mg/mL（quercetin concentration）,but didn＇t show obvious inhibitory effect on mould.The alcohol extract of lotus seed pod has antioxidant properties and antibacterial properties,and has positive correlation to the concentration of quercetin（R〉0.980 0,P〈0.05）.