马蹄多糖微波辅助提取工艺优化及其抗氧化活性研究

Study on microwave-assisted extraction and antioxidation of polysaccharides from water chestnut

以马蹄为原料,利用正交试验优化其多糖的微波辅助提取工艺,并对马蹄多糖的抗氧化性进行检测。结果表明：以蒸馏水为提取溶剂,马蹄多糖最佳提取工艺为微波功率600 W,料液比115（g/mL）,提取时间3 min,该条件下马蹄多糖提取率可达8.12%,纯度达83.2%。马蹄多糖有较强的还原力,在0.01~0.10 mg/mL质量浓度范围内与抗坏血酸相当;对羟自由基有较强的清除能力,在0.10 mg/mL时,马蹄多糖对羟自由基的清除率达到46.18%。

The water chestnut as raw material,the extraction conditions of polysaccharide were studied by single factor and orthogonal experiment,antioxidant activity on polysaccharide from water chestnut was detected,respectively.The results showed that with distilled water as extraction solvent,extracting process of polysaccharide from water chestnut had microwave power of 600 W,solid-liquid ratio of 1：15,extracting time of 3 min,under these conditions,the extraction rate of polysaccharide could reach 8.12%,and the polysaccharide purity could up to 83.2%.The polysaccharide from water chestnut had strong reduction power and scavenging ability to hydroxyl radicals in the range of 0.01 ~ 0.10 mg/mL mass concentration,and the removal rate of hydroxyl radical reached 46.18% when the concentration of polysaccharide was 0.10mg/mL.