不同工艺制备辣椒籽油的体外抗氧化活性比较

Comparing antioxidant activity of pepper seeds oil in vitro produced by different process

采用体外抗氧化活性试验模型,以Vc和VE作对照,比较研究超临界CO_2萃取、石油醚萃取及超临界CO_2萃取后脱色脱胶3种工艺制备的辣椒籽油的体外抗氧化活性。结果表明,不同工艺制备的辣椒籽油的还原能力以及对羟基自由基、超氧阴离子自由基和H_2O_2的清除率从高到低依次为超临界萃取后脱色脱胶、超临界萃取、石油醚萃取,对DPPH自由基清除率从高到低依次为石油醚萃取、超临界萃取、超临界萃取后脱色脱胶;超临界萃取后脱色脱胶、超临界萃取、石油醚萃取3种工艺制备的辣椒籽油对超氧阴离子自由基的IC_(50)分别为0.09,0.13,0.34mg/mL,对羟基自由基的IC_(50)分别为0.98,1.73,2.19 mg/mL,对DPPH自由基的IC_(50)分别为38.47,10.82,2.00 mg/mL;综合比较。

The antioxidant activity in vitro experimental model method is adopted,with control of Vc and VE,a comparative study of supercritical CO_2 extraction and petroleum ether extraction and supercritical CO_2 extraction after degumming and bleaching of three kinds of process technology for preparation of pepper seed oil antioxidant activity.The results show that the reduction ability of pepper seed oil and the hydroxyl radical,superoxide anion free radicals and H_2O_2 scavenging rate from high to low process followed by supercritical fluid extraction after degumming and bleaching,supercritical fluid extraction,petroleum ether extraction,DPPH free radical scavenging rate from high to low craft followed by petroleum ether extraction,supercritical fluid extraction,supercritical fluid extraction degumming and bleaching;Super critical fluid extraction after degumming and bleaching,supercritical fluid extraction,petroleum ether extraction of three kinds of technology to prepare the pepper seed oil on superoxide anion free radical IC_（50） respectively 0.09 mg/mL,0.13 mg/mL,0.34 mg/mL. The IC_（50） of hydroxyl radicals respectively for 0.98 mg/mL,1.73 mg/mL,2.19 mg/mL.The IC_（50） of DPPH free radical respectively for 38.47 mg/mL,10.82 mg/mL,2.00 mg/mL;It is showed that the three kinds of process,pepper seed oil using supercritical CO_2 extraction after decolorization of the best.