1型糖尿病患儿调节性B细胞变化初探

The changes of regulatory B cell subset in children with type 1 diabetes mellilus

以流式细胞术检测1型糖尿病患儿外周血调节性B细胞（ Breg） CD19＋CD24hi CD38hi Breg、CD4＋CD25＋Treg比例,酶联免疫吸附法检测培养上清中白细胞介素10（ IL-10）浓度；实时定量PCR检测外周血B细胞IL-10和CD4＋T细胞中Foxp3、细胞毒T淋巴细胞抗原4（ CTLA-4）、糖皮质激素诱导的肿瘤坏死因子受体（GITR）的mRNA表达。结果显示,1型糖尿病患儿外周血CD19＋CD24hiCD38hiBreg细胞比例、B细胞IL-10 mRNA和蛋白水平均明显低于对照组（P＆lt;0.05）；Treg相关分子Foxp3、CTLA-4和GITR mRNA表达明显低于对照组（P＆lt;0.05）。这些结果提示Breg细胞数量及功能异常可能是导致1型糖尿病患儿免疫功能紊乱的重要原因之一。

[Summary] The proportions of CD19＋CD24 hi CD38 hi regulatory B cells （ Breg ） and CD4＋CD25＋Treg in peripheral blood of children with type 1 diabetes mellitus （ T1DM） were detected by flow cytometric analysis. The concentration of interleukin 10 （IL-10） protein was measured by ELISA. The mRNA expression levels of Foxp3, cytotoxic T lymphocyte-associated antigen-4 （ CTLA-4 ） , and glucocorticoid-induced tumor necrosis factor receptor （ GITR） in CD4＋T cells and IL-10 in CD19＋ B cells were evaluated using real-time PCR. The results showed that the proportions of CD19＋CD24hiCD38hiBreg in peripheral blood, the mRNA and protein levels of IL-10 in B cells from patients with T1DM were lower than those from healthy controls （P〈0. 05）. The mRNA expression levels of Treg associated factors such as Foxp3, CTLA-4, and GITR were lower in CD4＋ T cells from children with T1DM compared with the controls（P〈0. 05）. These results suggest that Breg cell deficiency and dysfunction might be one of the important factors causing cellular immune dysfunction in patients with T1DM.