福建省番茄灰霉病菌对氟啶胺敏感基线建立及与不同杀菌剂的交互抗性

Baseline sensitivity of Botrytis cinerea to fluazinam and cross-resistance to different fungicides in Fujian province

为明确中国福建省番茄灰霉病菌对氟啶胺的敏感性及其与不同杀菌剂的交互抗性,采用菌丝生长速率法测定了106株采自福建省主要番茄产区的番茄灰霉病菌对氟啶胺的敏感性。结果表明,氟啶胺对福建省番茄灰霉病菌菌丝生长的EC50值在0.003 7~0.045 2μg/m L之间,平均值为（0.022 1±0.009 8）μg/m L,其敏感性频率分布呈连续单峰曲线,符合正态分布,因此可将该EC50平均值（0.022 1±0.009 8）μg/m L作为福建省番茄灰霉病菌对氟啶胺的敏感基线,用于其田间抗药性监测。从106株菌株中选择15株对氟啶胺敏感性不同的菌株,测定了其对嘧霉胺、异菌脲、腐霉利和啶氧菌酯的敏感性。结果表明,供试5种杀菌剂对15株番茄灰霉病菌菌丝生长的平均抑制活性依次为氟啶胺〉异菌脲〉腐霉利〉啶氧菌酯〉嘧霉胺,氟啶胺与异菌脲、腐霉利、啶氧菌酯和嘧霉胺之间均不存在交互抗性。

To evaluate the sensitivity of Botrytis cinerea to fluazinam and the existed cross-resistance between fluazinam and other fungicides, 106 single spore isolates of B. cinerea were collected from tomato in Fujian province. The sensitivities of B. cinerea isolates to fluazinam were determined by mycelial growth rate test. The results showed that EC50 values of 106 isolates to fluazinam followed a unimodal curve, ranging from 0.003 7 to 0.045 2 μg/m L with the mean EC50 value of（0.022 1 ±0.009 8） μg/m L. Thus, the data could be employed as the baseline-sensitivity of B. cinerea to fluazinam which could be used for the field resistance monitoring. And the sensitivities of 15 isolates of B.cinerea, which exhibited different sensitivities to fluazinam, to pyrimethanil, iprodione, procymidone and picoxystrobin, were also tested with the mycelial growth rate method. The mycelial growth inhibition rates of the above mentioned 5 fungicides were fluazinam iprodione procymidonepicoxystrobin 〉pyrimethanil. No cross-resistance between fluazinam and other 4 fungicides was observed.