拮抗多种植物病原真菌Streptomyces triostinicus C2的分离与鉴定

Isolation and Identification of Streptomyces triostinicus C2 Antagonizing on a Variety of Plant Pathogenic Fungi

旨在分离筛选获得对植物病原真菌具有抑制作用的拮抗菌。以水稻纹枯病菌为指示菌,采用平板对峙培养法进行拮抗菌的分离筛选;根据菌体形态学观察、生理生化特征以及16S r DNA序列分析,对拮抗菌进行菌种鉴定。结果显示,从采集自云南、广东、安徽、湖北、江西等地的18个土样中分离筛选到一株对水稻纹枯病菌具有良好抑制效果的菌株C2,该菌株初步鉴定为Streptomyces triostinicus,并暂命名为链霉菌C2;进一步测定链霉菌C2的抗菌谱发现,它对水稻纹枯病菌、水稻稻瘟病菌、葡萄炭疽病菌、西瓜枯萎病菌和橘青霉等多种植物病原真菌均具有良好的抑制作用,其抑菌率分别为49.78%、56.62%、80.96%、18.59%和94.10%。链霉菌C2是一株植物病原真菌广谱拮抗菌。

The research purposes of this work are to isolate and screen a strain against phytopathogenic fungi.The antagonistic strainwas isolated and screened using plate confrontation culture with Rhizoctonia solani as indicator,and was further identified on the basis ofmorphological characteristics,physiological and biochemical characterization,and 16 S rDNA sequence analysis.From 18 soil samplescollected from Yunnan,Guangdong,Anhui,Hubei,and Jiangxi Provinces of China,the isolated actinomycete strain C2 presented astrong inhibitory effect on R.solani,and it was preliminarily identified as Streptomyces triostinicus,designated as Streptomyces strain C2 here.The bioassay results showed that Streptomyces strain C2 possessed a broad-spectrum inhibitory effect on a range of plant fungi such as R.solani,Pyricularia grisea,Colletotrichum gloeosporioides,Fusarium oxysporum f.sp.niveum,and Penicillium citrinum,and the inhibitionpercentage was 49.78%,56.62%,80.96%,18.59%,and 94.10%,respectively.Conclusively,Streptomyces strain C2 was an antagonisticstrain with broad-spectrum in suppressing the growth of various plant fungal pathogens.