Act0988拮抗菌株鉴定及培养条件与菌株生长关系研究

Identification of Antagonistic Strain Act0988,and Relationships Between Cultivation Conditions and Its Growth

Act0988菌株产对柑橘青霉等多种霉菌具抗菌活性的代谢产物,对菌株进行鉴定、研究培养条件与菌株生长的关系,以为后续开发菌株产抗菌物质的培养基筛选及发酵工艺技术的研究奠定基础。以菌株的形态、培养特征、生理生化特征及16S rDNA序列分析进行菌种鉴定,三角瓶液体振荡培养研究碳氮源、温度、pH及氧与菌株生长的关系。结果表明,Act0988菌株为多产色链霉菌(Streptomyces polychromogenes),菌株生长最佳碳源为可溶性淀粉,发酵液生物量5.8 mg/mL;酵母膏与蛋白胨为最佳氮源,生物量5.7 mg/mL左右。菌株最适温度28℃,生物量6.0 mg/mL。最适初始pH7.0,生物量6.2 mg/mL;最适装液量60 mL/500mL,生物量6.3 mg/mL。Act0988菌株易培养,发酵液抗菌活性强,菌株产抗菌物质具有突出的开发利用潜力。

Strain Act0988 produces the metabolite with antifungal activity against many kinds of mould,such as Penicillium italicum etal.The strain identification and study of the relationship between cultivation condition and the strain growth were aimed at laying the foundationfor the screening media and research of fermentation processes during the development of the metabolite in future.The strain was identified by itsmorphological and culture characteristics,physiological and biochemical characteristics and 16 S r DNA sequencing.The relationships betweencarbon sources,nitrogen sources,temperature,pH,oxygen and strain growth were studied with shake flask cultivation.Strain Act0988 wasidentified as Streptomyces polychromogenes,the biomass of fermentative broth was 5.8 mg/mL if the optimal carbon source was soluble starch,the biomass was about 5.7 mg/mL while yeast extract and peptone were the optimal nitrogen sources,the biomass was 6.0 mg/mL when theoptimal temperature was 28℃,the biomass was 6.2 mg/mL while the most suitable initial pH was 7.0,and the biomass was 6.3 mg/mL if theoptimal volume of the medium was 60 mL per 500 mL shake flask.For being easy to be cultivated and fermentation broth owing high antifungalactivity,the metabolites produced by strain Act0988 has the great potential of development and utilization.