摘要
为探究阿魏菇醇溶性物质(PFECs)抗肿瘤功效,优化阿魏菇醇提物三萜类化合物(PFTPs)提取条件,获取最大提取率。采用MTT法检测PFECs对5种肿瘤细胞生长抑制活性;对两种正常细胞无明显抑制作用;采用超声辅助、微波辅助热水浸提法、单因素试验与响应曲面法相结合,对阿魏菇三萜类化合物提取工艺优化。结果显示,PFECs具有显著的抗肿瘤活性;PFTPs提取的最佳工艺参数为:料液比1∶19(g/mL)、提取温度75℃、提取时间22 min(超声40 min后),提取率为4.42%。
This work aims to explore the antitumor activity of alcohol soluble components from Pleurotus ferulae（PFECs）,and tooptimize the extraction condition of triterpenoids from P.ferulae（PFTPs）for maximum extraction yield.MTT was used to detect the inhibitoryactivity of PFECs to 5 kinds of tumor cells,no significant inhibition to 2 kinds of normal cells was observed.Ultrasonic-assisted,hot-waterleaching with ultrasonic-assisted,single-factor experiment,and response surface methodology were jointly employed to optimize the extractionprocess of PFTPS.The results showed that PFECs presented significant antitumor activity.The optimal process parameters for the PFTPsextraction were ：the ratio of ethanol to material 1∶19（g/mL）,extraction time 22 min and extraction temperature 7℃,and the extractingyield of PFTPs was 4.42%.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第7期206-216,共11页
Biotechnology Bulletin
基金
国家自然科学基金项目(31260267)
新疆自治区动物学重点学科资助(2011001)
新疆大学天山学者特聘教授科研项目资金
新疆大学博士启动资金项目(210-61287)
新疆生物资源基因工程重点实验室开放课题(XJDX0201-2014-08)
关键词
阿魏菇
醇提物
活性成分
抗肿瘤
三萜化合物
提取优化
响应曲面
Pleurotus ferulae
ethanol extracts
active components
antitumor activity
triterpenoids
optimization of extraction
response surface methodology
