新疆野生及栽培一枝蒿多糖对树突状细胞免疫功能的影响

Immunoregulative Action of Polysaccharides in Wild and Cultivated Artemisia rupestris in Xinjiang on Bone Marrow Dendritic Cells

研究和比较新疆野生与栽培一枝蒿多糖对骨髓来源树突状细胞(dendritic cells,DCs)成熟和功能的影响。超声法制备野生及栽培一枝蒿粗多糖;Sevage法除蛋白;蒽酮-硫酸法测定多糖含量;流式细胞术检测DCs的成熟;ELISA法检测IL-12和TNF-α的表达水平。结果显示,野生及栽培一枝蒿多糖含量分别为26.18%和22.14%,去除蛋白质后多糖含量分别为30.94%和27.06%;野生及栽培一枝蒿多糖均可以显著增强小鼠骨髓来源CD11c+DCs表面分子CD40,CD86及CD80的表达(P<0.05);显著促进IL-12和TNF-α的表达(P<0.05);显著降低DCs吞噬FITC-Dextran的能力;且相同剂量对DCs的免疫活性无显著性差异(P>0.05);除蛋白和不除蛋白的野生及栽培一枝蒿多糖的免疫活性均无显著差异(P>0.05)。新疆野生和栽培一枝蒿多糖含量差异较小,并均可以促进小鼠骨髓来源DCs的成熟和功能,且差异不大。

This work is to study and compare the effects of crude polysaccharides in wild Artemisia rupestris（WARCP）and cultivatedA.rupestris（CARCP）on the maturation and function of bone marrow dendritic cells（DCs）.The WARCP and CARCP were isolated byultrasonic extraction,the protein was removed by Sevage,the polysaccharides content of them were measured by anthrone-sulfuric acidmethod,the maturation of DCs were detected by flow cytometry,and the expressions of IL-12 and TNF-α were examined by ELISA.As results,the polysaccharides contents of WARCP and CARCP were 26.18% and 22.14% respectively,while the polysaccharides contents of WARCPand CARCP after removing protein were 30.94% and 27.06% respectively.Both WARCP and CARCP significantly enhanced the expressionlevel of CD40,CD86 and CD80 on the CD11 c ＋ DCs from mice＇s bone marrow（P 〈0.05）,significantly promoted the expression of IL-12 and TNF-α（P 〈0.05）,significantly reduced the capabilities of DCs phagocytosis to FITC-Dextran,and their immunoregulative actions atthe same dose of them was not significant（P〉 0.05）,there was no significant differences on immunoregulative action of WARCP and CARCPbetween removing and not removing protein（P 〉0.05）.In conclusion,there is little difference between WARCP and CARCP,and bothenhance the maturation and function of mouse＇s bone marrow DCs in little differencen.