离子色谱-电化学法检测化妆品中磺胺类物质

Determination of Sulfa Substance by Ion Chromatography-electrochemical Detection in Cosmetics

利用离子色谱分离电化学检测,建立了化妆品中磺胺类物质的检测方法。实验优化了影响磺胺类物质色谱分离因素,主要有柱温、淋洗液的配比、电极、电位和流速。结果表明,柱温35℃、淋洗液0.1 mol/L(14%)的KOH溶液、流速0.25 m L/min、Au电极和氨基酸电位(积分脉冲安培电位)、参比电极Ag/Ag Cl为分离9种磺胺类物质的最优条件。方法拥有良好的线性(r≥0.999 1)、检出限(≤1.0×10^(-2)mg/L)、重现性(RSD≤2.78%,n=8)及回收率(85.8%~113%),可用于检测复杂基体中的磺胺类化合物。

A method for determination of sulfa substances was developed by using ion chromatography with electrochemical detector. Some factors affecting the separation efficiency were optimized,such as column temperature,the ratio of eluent,electrode,potential and flow rate. The best conditions for separating 9 kinds of sulfa substances were 35 ℃ column temperature,0. 1 mol / L（ 14%） KOH,Au electrode,Ag/Ag Cl reference electrode,the amino acid potential（ integrated amperomet）,and 0. 25 m L/min flow rate. The method had good linear relationship（ r ≥0. 999 1）,low detection limit（ ≤1. 0 × 10^（-2）mg / L）,reproducibility（ RSD≤2. 78%,n = 8）,and high recovery（ from 85. 8% to 113%）. This method could be used to detect sulfa compounds in complex matrix sample.