金牛草药材质量标准研究

Research on quality standards of Aleuritopteris Herba

提高金牛草药材质量标准，为评价和控制金牛草药材质量提供依据。修订金牛草的性状及显微鉴别特征，参照《中国药典}2010年版附录相关方法，对金牛草药材的水分、总灰分、酸不溶性灰分及醇溶性浸出物进行测定。以粉背蕨素A和对照药材为对照，采用薄层色谱法进行定性鉴别；制备粉背蕨素A标准品，建立金牛草指标成分粉背蕨素A的含量测定方法。建立的性状、显微、薄层鉴别方法易于操作，具有较强的专属性；样品测定结果：水分8．8％～10．9％，总灰分7．6％～11．4％，酸不溶性灰分2．5％-4．2％，醇溶性浸出物9．3％-10．2％，粉背蕨素A质量分数0．56％-0．71％。通过完善金牛草的药材质量标准，能够更有效地评价其内在质量，保证用药品质。

This study aims to establish quality standards of Aleuritopteris Herba （AH）, which could supply scientific evidence for the quality control of AH. The morphological and microscopic identification characters were reformulated. The tests of water content, total ash, acid-insoluble ash and ethanol-soluble extractives of AH were carried out according to the methods recorded in appendix of Chinese Pharmacopeia （2010 edition, volume 1 ）. The TLC method was established by using aleuritopesis A [ 2,19-diol（2β,4α）-16- enekanreniod ] and reference herb as references. With preparation of aleuritopesis A [ 2,19-diol （ 2β,4α ） -16-enekaureniod ] reference substance, the content of aleuritopesis A in AH was determined by HPLC. As a result, the macroscopic identification, microscopic fea- tures and TLC methods were specific and simple. The water content, total ash, acid-insoluble ash and ethanol-soluble extractive and the content of aleuritopesis A of all samples varied in the ranges of 8. 8% -10. 9%, 7. 6% -11.4%, 2. 5% -4. 2%, 9.3% -10. 2% and 0. 56% - 0. 71%, respectively. The improved quality standard can be used to evaluate and guarantee the quality of AH comprehensively.