不同采血管、标本处理过程以及存放时间对NSE检测结果的影响

Effects of different vacuum tubes,specimen processing and storage time on the detection results of neuron specific enolase

目的探究不同采血管、标本处理过程以及存放时间对神经元特异性烯醇化酶(NSE)检测结果的影响。方法随机选取广东省中医院50例住院患者,空腹采静脉血,每份血液标本同时分装于2个试管,试管1为无添加剂干燥管(红管),试管2为促凝剂分离胶管(黄管),用罗氏E601全自动电化学发光免疫分析仪测定两管血清NSE水平。每管标本共测定3次,测定时间分别为标本采集后1、24、48h。其中1h和24h之间,标本经历了4℃保存24h、标本稍混匀和再离心的处理过程;24h和48h之间,标本只经历了4℃保存24h,随后直接上机检测NSE。结果红黄两管血清NSE水平在1h时结果,差异无统计学意义(P>0.05);红管在24h时结果的均差值及阳性率显著高于1h结果的均差值及阳性率,且差异有统计学意义(P<0.05);红管的48h结果的均差值及阳性率与24h相比,差异无统计学差异(P>0.05)。黄管标本的3次(1、24、48h)NSE测定结果的均差值及阳性率差异均无统计学意义(P>0.05)。结论促凝剂分离胶管(黄管)对NSE血清测定结果的稳定性优于无添加剂干燥管(红管)。

Objective To explore the effects of different vacuum tubes ,specimen processing and storage time on the detection re‐sults of neuron specific enolase （NSE） .Methods Totally 50 hospitalized patients were randomly selected .The venous blood of each patient was split into 2 tubes .One was drying tubes without additive （red tubes） and the other was separating gel tubes with coagu‐lator （yellow tubes） .NSE was then measured by ROCHE E601 .NSE of each tube was detected after 1 ,24 ,48 h of blood separa‐ting .Between 1 h and 24 h ,sample experienced storage （4 ℃ ,24 h） and specimen processing （ such as ：mixing slightly and centri‐fuge again） .However ,between 24 h and 48 h ,sample experienced only storage （4 ℃ ,24 h） .Results There was no significant difference in the NSE of 1 h for red and yellow tubes （P〉0 .05） .To red tubes ,concentration and positive rate of 24 h for NSE were significantly higher than that of 1 h（P〈0 .05） ,and there was no significant difference in 48 h and 24 h （P〉 0 .05） .To yellow tubes ,there was no significant differences in 1 ,24 ,48 h （P〉 0 .05） .Conclusion The stability of the coagulant separating tube （yellow tubes） was better than that of the non additive drying tube （red tube） for the determination of NSE in serum .