5例急性巨核细胞白血病患者免疫表型结果分析

Analysis of immunophenotype results in 5 patients with acute megakaryoblastic leukemia

目的通过对急性巨核细胞白血病患者白血病细胞免疫表型进行检测来分析各种血细胞表面分化抗原(CD)在急性巨核细胞白血病诊断及鉴别诊断当中的作用。方法应用BD公司流式细胞仪及其配套检测试剂盒检测5例急性巨核细胞白血病患者血细胞CD。结果所有患者均表达CD41和CD42,阳性率100%;淋系抗原均为阴性;CD13阳性者有4例,阳性率80%;CD33阳性者3例,阳性率60%;表达CD14和CD15者各1例,阳性率各占20%。结论检测结果显示CD41和CD42在急性巨核细胞白血病诊断及鉴别诊断中具有重要的价值,同时也再次验证了急性巨核细胞白血病患者血细胞存在有"系列非专一性"的特点。

Objective To analyze the role of various blood cell surface differentiation antigens（CD）in the diagnosis and differential diagnosis of acute megakaryoblastic leukemia by detecting the immunophenotype in 5patients with acute megakaryoblastic leukemia.Methods The flow cytometry produced by the BD company and its matching reagents kits were used to detect CD in 5patients with acute megakaryoblastic leukemia.Results All patients expressed CD41 and CD42with the positive rate of 100%;the lymphoid antigens ware negative in all cases;CD13was positive in 4cases with the positive rate of 80%;CD33was positive in 3cases with the positive rate of 60%;CD14and CD15 were expressed in each 1case,the positive rate was 20%.Conclusion The test results show that the CD41 and CD42have an important value in the diagnosis and differential diagnosis of acute megakaryoblastic leukemia.At the same time,the characteristic of ＂series non-specificity＂ in the blood cells of the patients with acute megakaryoblastic leukemia is verified again.