HPLC法同时测定参芪五味子片中毛蕊异黄酮葡萄糖苷、五味子酯甲和五味子甲素的含量

Simultaneous Determination of Calycosin-7-glucoside,Schisantherin and Deoxyschizandrin in Shenqi Wuweizi Tablets by HPLC

目的：建立HPLC法测定参芪五味子片中的毛蕊异黄酮葡萄糖苷、五味子酯甲和五味子甲素含量的方法。方法：采用色谱柱：Agilent ZORBAX SBC18（250 mm×4.6 mm,5μm）;以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱;流速：1.0 m L·min-1;检测波长：267 nm;柱温：28℃。结果：毛蕊异黄酮葡萄糖苷的进样量在0.11～2.75μg范围内（r=0.999 1）、五味子酯甲的进样量在1.05～26.25μg范围内（r=0.999 5）、五味子甲素的进样量在1.06～26.50μg范围内（r=0.999 4）线性关系良好,毛蕊异黄酮葡萄糖苷、五味子酯甲和五味子甲素的平均回收率分别为99.79%（RSD=1.43%,n=6）、99.37%（RSD=1.59%,n=6）和100.40%（RSD=2.11%,n=6）。结论：本方法简便快捷,重现性好,结果准确,适用于参芪五味子片的含量测定。

Objective： To establish a method for the determination of calycosin-7-glucoside,schisantherin and deoxyschizandrin in Shenqi Wuweizi Tablets. Methods： The separation was performed on a Agilent ZORBAX SBC18 column（ 250 mm × 4. 6 mm,5 μm） with acetonitrile-0. 1% phosphoric acid solution as mobile phase with gradient elution at the flow rate was 1. 0 m L·min-1. The detection wavelength was set at 267 nm and the column temperature was 28 ℃.Results： The linear range of calycosin-7-glucoside,schisantherin and deoxyschizandrin was 0. 11-2. 75 μg（ r = 0. 9991）,1. 05-26. 25 μg（ r = 0. 999 5） and 1. 06-26. 50 μg（ r = 0. 999 4）. The average recovery rates were 99. 79%（ RSD= 1. 43%,n = 6）,99. 37%（ RSD = 1. 59%,n = 6） and 100. 40%（ RSD = 2. 11%,n = 6）,respectively. Conclusion： The established HPLC method is proved to be convenient,reproducible,precise and suitable for the content determination of Shenqi Wuweizi Tablets.