RP-HPLC法同时测定胆石消胶囊中四种蒽醌类成分的含量

Determination of the contents of four types of anthraquinones in danshixi-ao capsules via RP-HPLC method

目的建立以RP—HPLC法同时测定胆石消胶囊中大黄酸、大黄素、大黄酚和大黄素甲醚的含量测定方法。方法采用Waters SunFire C18柱（250mm×4．6mm，5μm），以甲醇-0．1％磷酸（85：15）为流动相，流速：1．0ml／min，柱温：28℃，检测波长：254am。结果大黄酸、大黄素、大黄酚和大黄素甲醚进样量分别在0．13208～0．79248μg、0．08752～0．52512μg、0．11184～0．67104μg、0．08016～0．48096μg范围内与峰面积呈良好的线性关系，相关系数分别为0．9998、0．9996、0．9994、0．9997，平均加样回收率分别为99．32％、98．80％、99．05％、99．14％，RSD分别为0．76％（n=9）、1．31％（n=9）、1．41％（n=9）、0．94％（n=9）。结论本方法简便易行，专属性强，准确度高，重现性好，可作为胆石消胶囊质量控制方法。

Objective To establish the content determination method of rhein,emodin,chrysophanol and physcion in Danshixiao capsules via RP-HPLC method.Methods Waters SunFire Ciscolumn （250 min×4.6 mm,5 μm） was adopted, and methanol-0.1% phosphate （85：15） was selected as mobile phase,with flow rate：1.0 ml/min,column temperature：28℃, and determination wavelength：254 nm.Results Sample size of rhein,emodin,chrysophanol and physcion was within the range of 0.132 08-0.792 48 μg,0.087 52-0.525 12 μg,0.111 84-0.671 04 μg,and 0.080 16-0.480 96 μg respectively, which showed favorable linear relations with peak areas.The correlation coefficient was 0.9998,0.9996,0.9994 and 0.9997 respectively,the average recovery rate was 99.32%,98.80%,99.05%,99.14% respectively,and RSD was 0.76% （n=9）, 1.31% （n=9）,1.41% （n =90）,0.94% （n =9） respectively.Conclusion The method is easy to operate,with high specificity, high accuracy and good reproducibility,which is able to be taken as the quality control method of Danshixiao capsules.