葡萄籽原花青素对自发性高血压大鼠主动脉重构的改善作用研究

Effect of grape seed procyanidins on improving aortic remodeling in spontaneous hypertension rats

目的探讨葡萄籽原花青素(GSP)对自发性高血压大鼠(SHR)主动脉重构的改善作用。方法 8周龄雄性SHR 24只随机分为4组,每组6只:SHR组、GSP低剂量(50 mg/kg)、高剂量组(200 mg/kg)以及卡托普利组(30 mg/kg)。6只同龄雄性Wistar-Kyoto大鼠设为对照组。每周测定尾动脉收缩压(SBP);治疗6周后HE染色下测定胸主动脉中膜横截面积(MCSA)、管腔面积(LA)及MCSA/LA;Masson三色法测量胸主动脉胶原容积分数(CVF);ELISA法测定胸主动脉中Ⅰ型胶原(ColⅠ)含量;生化法检测大鼠胸主动脉中过氧化氢酶(CAT)活力及丙二醛(MDA)含量;蛋白免疫印迹法(Western blot)检测胸主动脉中ERK1/2蛋白表达。结果GSP能显著降低SHR SBP(P<0.01),改善血管重构参数,减少胸主动脉MDA含量以及ERK1/2蛋白的表达,提高CAT活力。结论 GSP能够改善SHR胸主动脉重构,其作用可能是通过降压、对抗氧化应激和抑制ERK1/2蛋白表达实现的。

Objective To investigate the effect of grape seed procyanidins （GSP） on improving aortic remodeling in spontaneous hypertension rats （SHR）. Methods Twenty-four male SHRs （8-week old） were randomized into 4 groups with 6 in each group： SHR group, low-dose GSP and high-dose GSP groups （50 or 200 mg/kg） and Captopril group （30 mg/kg）. Meanwhile, 6 Wistar Kyoto rats （8-week old） were served as control group. Tail systolic pressure was measured every week. After 6-week treatment, media cross-sectional area （MCSA）, lumen area （LA） and MCSA/LA of thoracic aorta were calculated after HE staining. Collagen volume fraction （CVF） was examined by Masson staining. ELISA and biochemical techniques were performed to determine the type I collagen （Col I）, malondiadehyde （MDA） and catalase （CAT） levels respectively. Western blot was used to detect the expression of ERK1/2 in thoracic aorta. Results GSP could significantly decrease tail systolic pressure （P 〈 0.01）, improve vascular remodeling parameters, reduce MDA content and the expression of ERK1/2 in thoracic aorta （P 〈 0.05）, but increase CAT activity （P 〈 0.05） after 6-week treatment. Conclusions GSP significantly improves thoracic aorta remodeling of SHR by lowering systolic pressure, inhibiting oxidative stress as well as suppressing the expression of ERK1/2.