特发性肺纤维化患者外周血T淋巴细胞亚群及相关细胞因子的变化

Changes of T lymphocytes subset and related lymphocytes cytokine in peripheral blood mononuclear cells of patients with idiopathic pulmonary fibrosis

目的探讨特发性肺纤维化(IPF)患者外周血中T淋巴细胞亚群和相关细胞因子的变化。方法选取35例IPF患者(IPF组)和38例健康体检人员(对照组),分别采用流式细胞技术及酶联免疫吸附法检测外周血CD3+、CD4+、CD8+T细胞百分率及血清干扰素γ(IFN-γ)、白介素(IL)-2、IL-4、IL-13的水平。结果 IPF组患者外周血CD3+、CD4+淋巴细胞亚群及CD4+/CD8+比值与对照组比较明显下降,CD8+明显增加,差异均有统计学意义(t分别=5.65、3.06、-6.10、3.35,P均<0.05)。IPF组患者外周血中IFN-γ和IL-2水平明显低于对照组,IL-4和IL-13水平明显高于对照组,差异均有统计学意义(t分别=6.13、4.03、-5.38、-8.28,P均<0.05)。结论 IPF患者外周血T淋巴细胞亚群及相关细胞因子发生变化,提示存在免疫系统紊乱,免疫机制可能参与了IPF的发病并在其中发挥重要作用。

Objective To explore the changes of T lymphocytes subset and related lymphocytes cytokine in peripheral blood mononuclear cells of patients with idiopathic pulmonary fibrosis（IPF）. Methods Thirty-five patients with IPF and38 healthy volunteers were selected to detect the percentages of CD^3＋, CD^4＋, CD^8＋T lymphocytes in the peripheral blood mononuclear cells by enzyme linked immunosorbent assay（ELISA） and the levels of interferon-γ（IFN-γ）,interleukin（IL）-2,IL-4,IL-13 in the blood serum by flow cytometry（FCM）. Results The lymphocytes subset（CD^3＋, CD^4＋） and the ratio of CD^4＋/CD^8＋in IPF group were significantly lower than those in the control group while the level of CD^8＋in IPF group was significantly higher（t=5.65,3.06,-6.10,3.35,P〈0.05）. The level of IFN-γ and IL-2 in IPF group were significantly lower than those in the control group,while levels of IL-4 and IL-13 in IPF group were significantly higher than those in the control group（t=6.13,4.03,-5.38,-8.28,P〈0.05）. Conclusion The changes of T lymphocytes subset and related lymphocytes cytokine in peripheral blood mononuclear cells of patients with idiopathic pulmonary fibrosis indicate that the im-mune system may participate in and play an important role in pathogenesis of the IPF.