16年生大叶相思高效芽诱导、增殖体系研究

Efficient bud inducing and proliferation of 16-year-old Acacia auriculiformis plant

【目的】充分利用大叶相思Acacia auriculiformis成年优树的优良性状,提高大叶相思优质苗木扩繁效率、加快其良种选育及推广。【方法】以16年生大叶相思为材料,对外植体消毒方式进行研究,结合外植体及芽诱导培养基的选择,建立大叶相思高效芽诱导体系;对增殖培养基进行筛选,并研究多次继代与增殖倍数的关系。【结果】于8月取当年生枝条第3~5腋芽的茎段(最优外植体),选择φ为0.1%升汞和75%乙醇分别处理18 min和15 s最优外植体,接种于最佳芽诱导培养基MS+6-BA 1.0 mg·L^(-1)+蔗糖40 g·L~(^(-1)),芽诱导率为92.00%。增殖培养基为II型培养基(MS+6-BA 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+Ac 0.05 g·L^(-1)+蔗糖30 g·L^(-1))时增殖倍数高且稳定,7次继代平均增殖倍数为2.63。【结论】建立的高效组培体系适于大叶相思成年优树的快速扩繁。

[ Objective ] To utilize the superior properties of mature Acacia auriculiformis, improve propa- gation efficiency, and accelerate breeding and promotion of elite varieties. [ Method ] A. auriculiformis bud inducing system was established by comparing the disinfection strategies for the explants from 16- year-old A. auriculiformis and screening the bud inducing media. The proliferation media were screened and the relationship between subculture times and proliferation rate was studied. [ Result ] The optimal explants were those collected from the 3th-5th node of shoots in August. For the optimal explants treated with 0.1% mercuric chloride for 18 min and 75% ethanol for 15 s, and inoculated onto the best bud inducing medium containing MS medium ＋ 6-BA 1.0 mg . L^-1 ＋ sucrose 40 g . L-1, the germination rate reached 92.00%. When type II medium （MS＋ 6-BA1.0 mg.L-1 ＋ NAA0.1 mg. L^-1 ＋Ac0.05 g . L^-1 ＋ sucrose 30 g . L^-1） was used for proliferation, the proliferation rate was high and stable with an average rate of 2.63 for seven generations. [ Conclusion ] This tissue culture system enables fast propagation for mature elite plants of A. auriculiformis.