高效液相色谱法测定婴幼儿配方乳粉中酪蛋白磷酸肽的含量

Determination of Casein Phosphopeptide in Infant Formula Milk Powder by High Performance Liquid Chromatography

实验建立婴幼儿配方乳粉中酪蛋白磷酸肽的高效液相色谱测定方法。采用超声提取,ODS色谱柱分离,流动相为0.1%乙酸水溶液和乙腈,梯度洗脱,紫外检测器检测。结果表明：方法检出限为6.25 mg/kg,线性范围为5.0~100.0μg/m L,加标回收率为71.4%~94.2%。该方法样品前处理简单,检测结果符合定性定量要求,可用于婴幼儿配方乳粉中酪蛋白磷酸肽的测定。

A method for the determination of casein phosphopeptide （CPP） in infant formula milk powder by high performance liquid chromatography （HPLC） was developed. Casein phosphopeptide was extracted with water by ultrasonic treatment. The analysis was performed on a ODS column using 0.1% acetic acid and acetonitrile as the mobile phase under gradient elution. The limit of detection of the method was 6.25 mg/kg, the calibration curve was linear between 5.0 and 100.0 μg/mL. Recoveries were between 71.4% and 94.2%. This method was simple and the results complied with the qualitative and quantitative requirements. It therefore can be used to detect casein phosphopeptide in infant milk powder.