在不同饲料中添加加热灭活的植物乳酸杆菌P-8对大菱鲆幼鱼抗氧化能力和血清生化指标的影响

Effect of supplementing heat-inactivated Lactobacillus plantarum P-8 to different feeds on anti-oxidative ability and blood biochemical properties of juvenile turbot(Scophthatmus maximus)

将植物乳酸菌P-8以灭活的形式添加到大菱鲆幼鱼的两种基础饲料中,旨在研究灭活植物乳酸杆菌(HK-LP)对大菱鲆饲料中豆粕替代鱼粉的影响。试验结束时取血测定血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、碱性磷酸酶(ALP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)活力和总蛋白(TP)、胆固醇(TCHO)、甘油三酯(TGK)含量。结果显示,D因素和P因素在AST、ALP、TGK和SOD有显著交互作用(P<0.05)。D因素ALT、TCHO、TP、TGK、MDA和SOD有显著性影响(P<0.05);P因素对SOD、ALP、AST、ALT、TGK和TP有显著性影响(P<0.05),且对血清生化指标ALP、AST、ALT、TGK和TP有极显著影响(P<0.01),血清TCHO、MDA呈下降趋势,但未达到显著水平(P>0.05),CAT呈上升趋势,但未达到显著水平(P>0.05)。由此得知,在豆粕替代45%鱼粉蛋白饲料中添加一定浓度的灭活乳酸杆菌P-8对大菱鲆幼鱼血清生化指标和抗氧化能力有显著性影响。

Both heat-- killed Lactobacillus plantarum P-- 8 （HK-- LP） and soybean （D） play important roles in health status of fish. Therefore, a feeding trial was conducted to determine the interactive effects of dietary HK--LP （P） and soybean （D） on serum biochemical indices and oxidative status of turbot for 70 days. Fishmeal groups was fed fish meal without substitution for soybean meal including SOR0, SOR7, SOR9~ $45R0, $45R7 and $45R9 were soybeanmeal groups fed soybean meal replacing 45% fish meal diet~ SOR0 was as control group. The experimental results are summarized as follows. A significant interaction was found between P and D on glutamic oxalacetic transaminase（AST） .alka- line phosphatase （ALP）.triglyceride （TGK） and the activity of superoxide dismutase （SOD） （P~ 0.05）. Dietary HK -- LP significantly affected SOD, ALP. AST. glutamic -- pyruvic transaminase （ALT）,TGK and total protein（TP）meanwhile dietary D altered ALT. cholesterol （TCHO）.TP. TGK.Malondialdehyde （MDA） and SOD（P^0.05）. Under the effect of factor P , TCHO and MDA level were on the decline, but did not affected significantly; CAT activity was on the rise, but the difference was not significant（P^O. 05）.